Miniature Fluorescence Microscopy for Imaging Brain Activity in Freely-Behaving Animals.
10.1007/s12264-020-00561-z
- Author:
Shiyuan CHEN
1
;
Zichen WANG
1
;
Dong ZHANG
2
;
Aiming WANG
3
;
Liangyi CHEN
1
;
Heping CHENG
1
;
Runlong WU
4
Author Information
1. State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, PKU-Nanjing Institute of Translational Medicine, Peking University, Beijing, 100871, China.
2. Academy of Advanced Interdisciplinary Study, Peking University, Beijing, 100871, China.
3. Department of Electronics, Peking University, Beijing, 100871, China.
4. State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, PKU-Nanjing Institute of Translational Medicine, Peking University, Beijing, 100871, China. rlwu@pku.edu.cn.
- Publication Type:Journal Article
- Keywords:
Brain imaging;
Miniature fluorescence microscopy;
Neuronal information processing;
Two-photon microscopy
- From:
Neuroscience Bulletin
2020;36(10):1182-1190
- CountryChina
- Language:English
-
Abstract:
An ultimate goal of neuroscience is to decipher the principles underlying neuronal information processing at the molecular, cellular, circuit, and system levels. The advent of miniature fluorescence microscopy has furthered the quest by visualizing brain activities and structural dynamics in animals engaged in self-determined behaviors. In this brief review, we summarize recent advances in miniature fluorescence microscopy for neuroscience, focusing mostly on two mainstream solutions - miniature single-photon microscopy, and miniature two-photon microscopy. We discuss their technical advantages and limitations as well as unmet challenges for future improvement. Examples of preliminary applications are also presented to reflect on a new trend of brain imaging in experimental paradigms involving body movements, long and complex protocols, and even disease progression and aging.
