Detection of a BRCA1 c.2013_2014ins GT variant an ethnic Han Chinese pedigree affected with breast cancer.
10.3760/cma.j.issn.1003-9406.2020.04.012
- Author:
Pan QI
1
;
Linlin GAO
;
Xiaoying HE
;
Yuehan NI
;
Sheng XU
;
Xueying MAI
;
Guiling ZHANG
;
Yuxia LIU
;
Yu GUO
;
Yong ZHOU
;
Qingtao HU
Author Information
1. Department of Head, Neck and Breast Surgery, Xinxiang Central Hospital, Henan 453000, China. huqingtao@nibs.ac.cn.
- Publication Type:Case Reports
- MeSH:
Asian Continental Ancestry Group;
BRCA1 Protein;
genetics;
Breast Neoplasms;
genetics;
Exome;
Female;
High-Throughput Nucleotide Sequencing;
Humans;
Pedigree;
Phenotype
- From:
Chinese Journal of Medical Genetics
2020;37(4):415-418
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To detect potential variant in an ethical Han Chinese pedigree affected with breast cancer.
METHODS:The proband and her relatives were subjected to next-generation sequencing using a target capture sequencing kit containing 121 cancer-related genes. Candidate variants were selected by analysis of their type, frequency in population, and segregation with the phenotype. Candidate variant was verified by Sanger sequencing and TA cloning.
RESULTS:A c.2013_2014ins GT variant was detected in the BRCA1 gene among all breast cancer patients from this pedigree but not among healthy females. The variant was not recorded in the 1000 Genome Project database or the Exome Aggregation Consortium (ExAC) database. The frameshifting insertion was predicted to form an premature stop codon in gene transcript and can give rise to a truncated protein.
CONCLUSION:The BRCA1 c.2013_2014ins GT variant probably underlies the pathogenesis of breast cancer in this Chinese pedigree.
