Identification and attribution of chemical constituents of Qingfei Paidu Decoction based on UHPLC-LTQ-Orbitrap-MS technology.
10.19540/j.cnki.cjcmm.20200423.202
- Author:
Yan-Yan ZHOU
1
;
Wen-Ya GAO
1
;
Xin-Ru GU
1
;
Zhou-Quan CHEN
2
;
Hai-Yu ZHAO
1
;
Bao-Lin BIAN
1
;
Li-Xin YANG
1
;
Nan SI
1
;
Hong-Jie WANG
1
;
Ying TAN
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. China Resources Sanjiu Medical & Pharmaceutical Co., Ltd. Shenzhen 518110, China.
- Publication Type:Journal Article
- Keywords:
Qingfei Paidu Decoction;
UPLC-LTQ-Orbitrap-MS;
chemical constituent identification;
component attribution
- MeSH:
Chromatography, High Pressure Liquid;
Coumarins;
analysis;
Drugs, Chinese Herbal;
Flavonoids;
analysis;
Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(13):3035-3044
- CountryChina
- Language:Chinese
-
Abstract:
UHPLC-LTQ-Orbitrap-MS was developed for the identification of chemical constituents in Qingfei Paidu Decoction, which will clarify its material basis. ACQUITY UHPLC HSS T3 chromatography column(2.1 mm×100 mm, 1.8 μm) was used with 0.1% formic acid(B)-acetonitrile(A) as the mobile phase in gradient elution. The decoction was detected by high-resolution liquid chromatography-mass spectrometry equipped with an ESI ion source in positive and negative mode. Based on the accurate mass measurements, retention time, mass fragmentation patterns combined with comparison of reference and literature reports, a total of 87 major compounds including 43 flavonoids, 9 alkaloids, 4 triterpenoid saponins, 1 sesquiterpene, 2 coumarins, 10 phenolic acids and 18 other compounds were tentatively screened and characterized. UHPLC-LTQ-Orbitrap-MS was employed to comprehensively elucidate the chemical components in Qingfei Paidu Decoction, which basically covered 20 Chinese medicines except gypsum in Qingfei Paidu Decoction. These collective results provide a scientific basis for further research on the quality control standard of Qingfei Paidu Decoction.
