Discussion on research idea of quality marker of Salvia miltiorrhiza based on biosynthetic pathway of tanshinone compounds.
10.19540/j.cnki.cjcmm.20200506.105
- Author:
Rui-Qian ZHANG
1
;
Xiao-Yi WU
1
;
Tian-Yuan HU
2
;
Ya-di SONG
1
;
Wei GAO
2
;
Lu-Qi HUANG
3
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
2. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China School of Pharmaceutical Sciences, Capital Medical University Beijing 100069, China.
3. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Chinese Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza;
biosynthetic pathway;
ferruginol;
hairy root;
miltiradiene;
quality marker(Q-marker);
tanshinones
- MeSH:
Abietanes;
Biosynthetic Pathways;
Plant Roots;
Salvia miltiorrhiza
- From:
China Journal of Chinese Materia Medica
2020;45(13):3098-3103
- CountryChina
- Language:Chinese
-
Abstract:
Based on the theory of Q-marker, the hairy root of Salvia miltiorrhiza and S. miltiorrhiza in many provinces were studied. The relative expressions of SmCPS, SmKSL and CYP76AH1 genes in hairy roots were detected by real-time fluorescence quantitative PCR and the contents of tanshinoneⅡ_A, cryptotanshinone, tanshinoneⅠ, 1,2-dihydrotanshinone, ferruginol and miltiradiene were detected by UPLC and GC-MS, respectively. Statistical analysis shows as fllows: in the hairy root of S. miltiorrhiza, the content of miltiradiene and ferruginol is positively correlated with the content of tanshinone compounds in the downstream, and the relative expression of important genes in the biosynthetic pathway of tanshinone can reflect the content of tanshinone compounds to a certain extent; in many provinces of S. miltiorrhiza, the content of ferruginol and tanshinone compounds can also be found that there is a positive correlation between the contents. Based on the biosynthetic pathway of tanshinone compounds, which is a special index component in S. miltiorrhiza, this study focused on the important relationship between the upstream gene, the middle intermediate compound and the downstream tanshinone compound content of the biosynthetic pathway, and explored the possible research ideas of improving the quality marker system of S. miltiorrhiza, and then provided the possible research ideas for understanding and studying the quality marker of traditional Chinese medicine from the biosynthetic pathway.
