Genome-wide identification and expression analysis of CSLA gene family of Dendrobium catenatum.
10.19540/j.cnki.cjcmm.20200506.103
- Author:
Ya-Qian GAO
1
;
Xue-Liang CHEN
1
;
Dong-Hong CHEN
1
;
Jing-Jing LIU
1
;
Jin-Ping SI
1
Author Information
1. State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University Lin'an 311300, China.
- Publication Type:Journal Article
- Keywords:
CSLA;
Dendrobium catenatum;
gene expression;
glucomannan;
stress treatment
- MeSH:
Basidiomycota;
Cold Temperature;
Dendrobium;
genetics;
Gene Expression Regulation, Plant;
Genome, Plant;
Multigene Family;
Phylogeny;
Plant Proteins;
genetics;
Stress, Physiological;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2020;45(13):3120-3127
- CountryChina
- Language:Chinese
-
Abstract:
Glucomannan is the key active ingredient of Dendrobium catenatum, and CSLA family is responsible for glucomannan biosynthesis. In order to systematically evaluate the CSLA family members of D. catenatum, the bioinformatics methods were performed for genome-wide identification of DcCSLA gene family members through the genomic data of D. catenatum downloaded from the NCBI database, and further analyses of their phylogenetic relationship, gene structure, protein conserved domains and motifs, promoter cis-elements and gene expression profiles in response to stresses. The results showed that D. catenatum contains 13 CSLA members, all of which contain 9-10 exons. In the evolutionary relationship, CSLA genes were clustered into 5 groups, DcCSLA genes were distributed in all branches. Among which the ancestral genes of groupI existed before the monocot-dicot divergence, and groupⅡ-Ⅴ only existed in the monocot plants, indicating that group Ⅰ represents the earliest origin group. CSLA proteins are characteristic of the signature CESA_CaSu_A2 domain. Their promoter regions contain cis elements related to stresses and hormones. Under different stress treatments, low temperature induces the expression of DcCSLA5 and inhibits the expression of DcCSLA3. Infection of Sclerotium delphinii inhibits DcCSLA3/4/6/8/9/10 expression. Under the treatment of jasmonic acid, DcCSLA11 expression was significantly up-regulated, and DcCSLA2/5/7/12/13 were significantly down-regulated. These results laid a foundation for further study on the function of DcCSLA genes in glucomannan biosynthesis and accumulation.
