Extraction technology, composition analysis and antioxidant and antimicrobial activities of volatile oil from fenugreek leaves.
10.19540/j.cnki.cjcmm.20200523.301
- Author:
Yan-Feng HE
1
;
Rui-Nan WANG
1
;
Lu-Lu ZHANG
2
;
Yi WANG
1
;
Peng-Cheng LIN
1
Author Information
1. College of Pharmacy, Qinghai Nationalities University Xining 810007, China Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province Xining 810007, China.
2. Qinghai University for Nationalities Law School Xining 810007, China.
- Publication Type:Journal Article
- Keywords:
GC-MS;
antimicrobial;
antioxidant;
fenugreek leaves;
supercritical CO_2 extraction;
volatile oil
- MeSH:
Antioxidants;
Distillation;
Oils, Volatile;
Plant Leaves;
Trigonella
- From:
China Journal of Chinese Materia Medica
2020;45(13):3161-3168
- CountryChina
- Language:Chinese
-
Abstract:
To define the extraction process, main components and antioxidative and antimicrobial activities of volatile oil from fenugreek(Trigonella foenum-graecum) leaves and its active substance basis. Response surface methodology was used for optimum supercritical CO_2 extraction conditions of essential oil from fenugreek leaves. The main components of volatile oil were analyzed by GC-MS, its antioxidant activity was evaluated by measuring the scavenging ability of 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, ABTS) free radical, and the antimicrobial effect of volatile oil was evaluated by K-B paper AGAR diffusion method. The results showed that the optimal extraction temperature was 50 ℃, the extraction time was 89 min, and the extraction pressure was 35 MPa. Under the conditions, the optimum extracting yield of volatile oil was 1.72%,which was about 1.5 times higher than that of the conventional steam distillation. A total of 52 compounds were found based on reference substance retention time and GC-MS fragmentation information or the existing literatures, and the major compounds were oleic acid(9.65%), carveol(9.41%), n-hexadecanoic acid(9.1%), linoleic acid(6.95%), methyl linolenate(5.4%), petroselinic acid(5.3%), testosterone(3.4%), sotolon(1.75%). The volatile oil of fenugreek showed moderate antioxidant activities in DPPH assay(IC_(50) value of 0.473 mg·mL~(-1)) and ABTS test(IC_(50) value of 0.107 mg·mL~(-1)). The oil had a stronger antimicrobial activity in vitro. MIC of the volatile oil ranged from 0.375 to 1.5 mg·mL~(-1). The results showed that the optimized volatile oil extraction process was stable, and the extraction yield was high. Fenugreek leaves contained a variety of volatile components, with obvious antioxidant and antibacterial activities. This study provides a certain theoretical basis for the comprehensive development and utilization of fenugreek.
