Study on metabolites of Laportea bulbifera extract in rat feces based on UHPLC-Q-TOF-MS~E technique.
- Author:
Dan WU
1
;
Ying LI
1
;
Si-Ying CHEN
1
;
Juan TANG
1
;
Yue-Ting LI
1
;
Jie PAN
1
;
Yong HUANG
1
;
Lin ZHENG
1
;
Ai-Min WANG
1
;
Yong-Jun LI
1
;
Yan-Yu LAN
1
;
Zi-Peng GONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Laportea bulbifera extract; UHPLC-Q-TOF-MS~E; caffeoquinic acid; flavonoids; metabolites
- MeSH: Administration, Oral; Animals; Chromatography, High Pressure Liquid; Feces; Plant Extracts; Rats; Rats, Sprague-Dawley; Urticaceae
- From: China Journal of Chinese Materia Medica 2020;45(13):3220-3227
- CountryChina
- Language:Chinese
- Abstract: This project is to study the metabolites of Laportea bulbifera extract in rat feces. After the SD rats were gavaged with the extract(136 g·kg~(-1), according to the crude drug dose), the metabolites in their feces were detected by UHPLC-Q-TOF-MS~E technique, and the obtained mass spectrometry data was combined with UNIFI software for prediction. The prototype components and metabolites in rat feces were identified with reference materials and related literature. A total of 43 metabolites were identified(including 8 prototype components and 35 metabolites). The metabolic pathways mainly include monocaffeoylquinic acid(hydrogenation reduction, ring-opening cracking, sulfation, hydroxylation, glucuronidation), quercetin(O-C2 bond ring-opening cleavage, C2-C3 double bond reduction, rutin carbonylation) and so on. The metabolites and metabolic process of L. bulbifera extract in rat feces were clarified, which provided a basis for the study of the active substances and its mechanism of action.
