Study on rapid quality analysis method of Shengxuebao Mixture.
10.19540/j.cnki.cjcmm.20200221.307
- Author:
Jian-Yang PAN
1
;
Zi-Mei ZHOU
1
;
Hai-Bin QU
1
;
Hong-Na LIU
2
;
Sheng-Xiang LIU
2
;
Yong-Jian SU
2
;
Shan-Shan HU
2
Author Information
1. Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China.
2. Qinghua Deren Xi'an Xingfu Co., Ltd. Xi'an 710043, China.
- Publication Type:Journal Article
- Keywords:
NIR spectroscopy;
Shengxuebao Mixture;
UV-Vis spectroscopy;
rapid analysis technology
- MeSH:
Calibration;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Least-Squares Analysis;
Mass Spectrometry;
Spectroscopy, Near-Infrared
- From:
China Journal of Chinese Materia Medica
2020;45(9):2115-2121
- CountryChina
- Language:Chinese
-
Abstract:
A rapid analysis method based on ultraviolet-visual(UV-Vis) spectroscopy, near infrared(NIR) spectroscopy and multivariable data analysis was established for quality evaluation of Shengxuebao Mixture. The contents of eight active ingredients of Shengxuebao Mixture including albiflorin, paeoniflorin, 2, 3, 5, 4'-tetra-hydroxy-stilbene-2-O-β-D-glucopyranoside, specnuezhenide,ecliptasaponin D, emodin, calycosin-7-glucoside and astragaloside Ⅳ were simultaneously detected by using this method. HPLC-UV-MS was used as a reference method for determining the contents of these ingredients. Partial least squares(PLS) analysis was implemented as a linear method for multivariate models calibrated between UV spectrum/NIR spectrum and contents of 8 ingredients. Finally, the performance of the model was evaluated by 24 batches of test samples. The results showed that both UV-Vis and NIR models gave a good calibration ability with an R~2 value above 0.9, and the prediction ability was also satisfactory, with an R~2 value higher than 0.83 for UV-Vis model and higher than 0.79 for NIR model. The overall results demonstrate that the established method is accurate, robust and fast, therefore, it can be used for rapid quality evaluation of Shengxuebao Mixture.
