A new cytotoxic isoflavane from Dalbergiae Odoriferae Lignum.
10.19540/j.cnki.cjcmm.20200107.202
- Author:
Man-Man ZHU
1
;
Hui WANG
2
;
Cheng-Neng MI
2
;
Wen-Li MEI
2
;
Cui-Juan GAI
2
;
Hao-Fu DAI
2
;
Miao YU
2
;
Xing-Quan WU
3
;
Hao WANG
2
Author Information
1. College of Bioengineering, Henan University of Technology Zhengzhou 450046, China Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101, China Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine Haikou 571101, China.
2. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences Haikou 571101, China Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine Haikou 571101, China.
3. College of Bioengineering, Henan University of Technology Zhengzhou 450046, China.
- Publication Type:Journal Article
- Keywords:
Dalbergiae Odoriferae Lignum;
chemical constituents;
cytotoxicity
- MeSH:
Antineoplastic Agents;
Chromatography, High Pressure Liquid;
HeLa Cells;
Humans
- From:
China Journal of Chinese Materia Medica
2020;45(9):2122-2129
- CountryChina
- Language:Chinese
-
Abstract:
Fourteen compounds were isolated from the ethanol extract of Dalbergiae Odoriferae Lignum by various chromatographic techniques, including column chromatographies on silica gel, Sephadex LH-20 and semi-preparative HPLC. Their structures were determined by spectroscopic techniques as S-3'-hydroxy-7,2',4'-trimethoxyisoxane(1), 2-(2',4'-dimethoxyphenyl)-6-hydroxybenzofuran(2), 2-(2'-hydroxy-4'-methoxyphenyl)-6-methoxybenzofuran(3), 7,2',4'-trimethoxydihydroisoflavone(4), sativanone(5), 3,9-dimethoxy-6H-benzofuro[3,2-c]chromen-6-one(6),(6 aS,11 aS)-homopterocarpin(7),(6 aS,11 aS)-8-hydroxy-3,9-dimethoxypterocarpan(8),(6 aS,11 aS)-3,8,9-trimethoxypterocarpan(9), isodalbergin(10), isoliquiritigenin(11), butein(12), butin(13) and 3,7,4'-trihydroxyflavone(14). Among them, compound 1 was a new compound, while 2 and 3 were new natural products, 6, 8, 9 and 14 were isolated for the first time from Dalbergiae Odoriferae Lignum. Compounds 1-14 were tested for their cytotoxic activity against human hepatoma cell line BEL-7402, human gastric cancer cell line SCG-7901, human lung cancer cell line A549, human chronic myeloid leukemia cell line K562 and HeLa human cervical cancer cellline by MTT method. Compound 1 exhibited significant cytotoxicity with IC_(50) values ranging from 2.85 to 11.62 μg·mL~(-1). In addition, 2, 11 and 12 showed weak cytotoxic activities.
