Simultaneous determination of 6 main components in Achilleae Herba by HPLC.
10.19540/j.cnki.cjcmm.20200220.201
- Author:
Meng XIAO
1
;
Zong-Yang LIU
1
;
Xia WU
1
;
Xiao-Qing CHEN
1
;
Ke ZAN
2
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
2. National Institutes for Food and Drug Control Beijing 100050, China.
- Publication Type:Journal Article
- Keywords:
Achilleae Herba;
HPLC;
caffeoylquinic acids;
content determination;
neopellitorine B;
pellitorine
- MeSH:
Chlorogenic Acid;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Principal Component Analysis
- From:
China Journal of Chinese Materia Medica
2020;45(9):2138-2143
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to establish an HPLC method for the simultaneous determination of 6 main components, including chlorogenic acid, 3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic acid, pellitorine and neopellitorine B in Achil-leae Herba. HPLC analysis was performed on a Merck Purospher STAR RP-18 endcapped(4.6 mm×250 mm, 5 μm), with a mobile phase consisting of 0.05% phosphoric acid solution(A)-acetonitrile(B) at a flow rate of 1 mL·min~(-1)(0-7 min,12%-14% B;7-10 min,14%-17% B;10-25 min,17%-22% B;25-35 min,22%-35% B;35-51 min,35%-80% B;51-60 min,80%-90% B). The detection wavelength was 254 nm and the column temperature maintained at 30 ℃, and the injection volume was 5 μL. The standard curves revealed a good linear relationship. The contents of 6 components were 0.404%-2.116% for chlorogenic acid, 0.160%-0.892% for 3,4-dicaffeoylquinic acid, 0.608%-1.464% for 3,5-dicaffeoylquinic acid, 0.168%-0.868% for 4,5-dicaffeoylquinic acid, 0.122%-1.234% for pellitorine, 0.065%-0.312% for neopellitorine B, respectively. Both cluster and principal component analysis can distinguish the research data in anthesis and pre-anthesis by software Chempattern. There were obviously differences in the different harvest time. Therefore, attention should be paid to the harvesting time of the herb. The method can be used to determine the contents of six main components, and can provide reference for the improvement of quality standard of Achilleae Herba.
