Effects of Renshenjian Decoction on blood and urine metabonomics in type 2 diabetes rats based on ~1H-NMR.
10.19540/j.cnki.cjcmm.20200219.201
- Author:
Meng-Qiu TANG
1
;
Wei-Dong LIN
1
;
Ming-Hua XIAN
1
;
Yuan-Yuan XIE
1
;
Yong-Xia YANG
2
;
Shu-Mei WANG
1
Author Information
1. Guangdong Pharmaceutical University Guangzhou 510006, China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica, State Administration of Traditional Chinese Medicine Guangzhou 510006, China Chinese Medicine Quality Engineering Technology Research Center of Guangdong Province/Higher-Education Guangzhou 510006, China.
2. Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
Renshenjian Decoction;
metabonomics;
type 2 diabetes;
~1H-NMR
- MeSH:
Animals;
Blood Glucose;
Diabetes Mellitus, Experimental;
Diabetes Mellitus, Type 2;
Metabolomics;
Proton Magnetic Resonance Spectroscopy;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2020;45(9):2186-2192
- CountryChina
- Language:Chinese
-
Abstract:
Proton nuclear magnetic resonance(~1H-NMR) is used to investigate the effect of Renshenjian Decoction on serum and urine metabolism of type 2 diabetic rats with insulin resistance induced by high-sugar and high-fat diet combined with low-dose streptozotocin(STZ). After the successful establishment of the insulin resistance model of type 2 diabetes, administration for 35 days, the serum and urine of rats were taken. Once the ~1H-NMR data have been collected and processed, PCA and OPLS-DA were used to analyze them. The results show that: compared with the blank group, the contents of methionine, taurine, α-glucose and β-glucose in the serum of the model group increased significantly(P<0.001), while the contents of 3-hydroxybutyric acid, lactic acid and unsaturated fatty acids decreased significantly(P<0.01). In the model group, the contents of trimethylamine oxide, glycine, α-glucose, β-glucose, taurine and phosphocholine in urine increased significantly(P<0.05), while the contents of creatine, lactic acid, acetic acid and citric acid decreased significantly(P<0.05). Compared with the model group, the contents of 3-hydroxybutyric acid and unsaturated fatty acids in serum of rats in the treatment group increased significantly(P<0.05), while the contents of taurine, α-glucose and β-glucose decreased significantly(P<0.01). In the treatment group, the contents of lactic acid, taurine and creatine in urine increased significantly(P<0.05), while the contents of trimethylamine oxide, glycine, α-glucose, β-glucose and phosphocholine decreased significantly(P<0.01). The results show that Renshenjian Decoction can regulate metabolic disorder and promote the metabolic phenotype to return to the normal range. It displayed therapeutic effect on type 2 diabetic rats with insulin resistance and provided a certain scientific basis for the biological basic research of Renshenjian Decoction by improving insulin resistance in diabetes mellitus.
