Analysis of bacteria distribution characteristics in different layers of agarwood based on Hiseq sequencing.
10.19540/j.cnki.cjcmm.20200302.115
- Author:
Xiang ZHANG
1
;
Jia-Qi GAO
2
;
Yuan YUAN
3
;
Quan YANG
1
;
Liang-Yun ZHOU
1
;
Juan LIU
3
;
Lu-Qi HUANG
4
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
2. School of Pharmacy, Jiangsu University Zhenjiang 212013, China.
3. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
4. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Aquilaria sinensis;
agarwood different layers;
bacterial community structure;
bacterial species diversity
- MeSH:
Bacteria;
High-Throughput Nucleotide Sequencing;
Oils, Volatile;
Thymelaeaceae;
genetics
- From:
China Journal of Chinese Materia Medica
2020;45(10):2374-2381
- CountryChina
- Language:Chinese
-
Abstract:
To explore the diversity of bacterial community structure between different layers of agarwood, Hiseq(high-throughput sequencing) was used to analyze the bacterial community structure of samples from different layers of agarwood. Our results showed that 1 150 096 optimized sequences and 9 690 OTUs were obtained from 15 samples of 5 layers of agarwood, which belonged to 28 bacterial phyla, 61 classes, 110 orders, 212 families and 384 genera. Further analysis revealed that the normal layer(NL) had the lowest bacterial species richness and the smallest number of OTUs. And the total number of OTUs of the agarwood layer(AL) and NL was zero, which was quite different.At the same time, there were significant differences in bacterial community structure and species diversity between NL and the other four layers. While there were some common dominant bacterial genera in both transition layer(TL) and NL. The similarity of bacterial distribution in 4 non-NL layers was relatively high, which had four common genera, such as Acidibacter, Bradyrhizobium, Acidothemus and Sphingomonas. While Acidibacter, Bradyrhizobium and Acidothemus were the dominant bacterial genus of DA and AL, and all of these layers contained volatile oil. In addition, the Bradyrhizobium was the most abundant in agarwood layer. Our results showed that bacterial community diversity and abundance were decreasing from DL to AL, and different layers showed significant differences in bacterial enrichment. It provided the clues to investigate how bacteria participate in the formation of agarwood.
