Characterization of inthomycin biosynthetic gene cluster revealing new insights into carboxamide formation.
10.1016/S1875-5364(20)60006-1
- Author:
Yong-Jiang WANG
1
,
2
;
Li WANG
1
,
2
;
Xin HE
1
,
2
;
Dong-Dong XU
1
,
2
;
Jun TANG
1
,
2
;
Ya-Tuan MA
3
;
Yi-Jun YAN
4
;
Yu-Cheng GU
5
;
Jing YANG
6
;
Sheng-Xiong HUANG
7
Author Information
1. State Key Laboratory of Phytochemistry and Plant Resources in West China, and CAS Center for Excellence in Molecular Plant Sciences, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
2. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China.
3. College of Chemistry and Pharmacy, Northwest Agriculture and Forestry University, Yangling 712100, China.
4. State Key Laboratory of Phytochemistry and Plant Resources in West China, and CAS Center for Excellence in Molecular Plant Sciences, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
5. Syngenta Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, U.K.
6. State Key Laboratory of Phytochemistry and Plant Resources in West China, and CAS Center for Excellence in Molecular Plant Sciences, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: yangjingc@mail.kib.ac.cn.
7. State Key Laboratory of Phytochemistry and Plant Resources in West China, and CAS Center for Excellence in Molecular Plant Sciences, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: sxhuang@mail.kib.ac.cn.
- Publication Type:Journal Article
- Keywords:
Biosynthesis;
Carboxamide;
Gene cluster;
Inthomycins;
P450
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(9):677-683
- CountryChina
- Language:English
-
Abstract:
Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B (1) and its two new analogues 2 and 3 were isolated from the crude extract of Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed via an oxidative release of the assembly chain assisted by the P450 domain.
