Novel SNP markers on ginsenosides biosynthesis functional gene for authentication of ginseng herbs and commercial products.
10.1016/S1875-5364(20)60017-6
- Author:
Wen-Ru WU
1
,
2
;
Chun-Song CHENG
3
;
Qi-Qing CHENG
3
;
Chi-Chou LAO
3
;
Hao CUI
3
;
Zi-Yu TANG
3
;
Yue OUYANG
3
;
Liang LIU
3
;
Hua ZHOU
1
,
4
Author Information
1. State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Macao 999078, China
2. School of Chinese Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
3. State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Macao 999078, China.
4. Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of China, Macau University of Science and Technology, Macao 999078, China. Electronic address: hzhou@must.edu.mo.
- Publication Type:Journal Article
- Keywords:
Dammarenediol synthase;
Nested PCR;
Panax ginseng;
Panax quinquefolius;
SNP;
UHPLC-TOF/MS
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(10):770-778
- CountryChina
- Language:English
-
Abstract:
Panax ginseng and Panax quinquefolius have similar bioactive components and morphological characteristics, but they are known to have different medicinal values, high-sensitive and accurate method is expected to identify the sources of ginseng products and evaluate the quality, but with a huge challenge. Our established UHPLC-TOF/MS method coupled with orthogonal partial least squares discriminant analysis (OPLS-DA) model based on 18 ginsenosides was applied to discriminate the sources of raw medicinal materials in ginseng products, and nested PCR strategy was used to discover 6 novel single nucleotide polymorphism (SNP) sites in functional dammarenediol synthase (DS) gene for genetic authentication of P. ginseng and P. quinquefolius for the first time. OPLS-DA model could identify the sources of raw ginseng materials are real or not. SNP markers were applied to identify ginseng fresh samples as well as commercial products, and proved to be successful. This established molecular method can tell exact source information of adulterants, and it was highly sensitive and specific even when total DNA amount was only 0.1 ng and the adulteration was as low as 1%. Therefore, this study made an attempt at the exploration of new type SNP marker for variety authentication and function regulation at the same time, and the combination of chemical and molecular discrimination methods provided the comprehensive evaluation and authentication for the sources of ginseng herbs and products.
