Correlation between differential expression of microRNA and quality of embryos.
10.3760/cma.j.cn511374-20190516-00244
- Author:
Mengxia NI
1
;
Yongfeng XUE
;
Jie DING
;
Shenmin YANG
;
Aiyan ZHENG
;
Yan PU
;
Wei WANG
;
Hong LI
;
Jian OU
Author Information
1. Reproductive and Genetic Center, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou Municipal Hospital, Suzhou, Jiangsu 215002, China. oj_1981@163.com.
- Publication Type:Journal Article
- From:
Chinese Journal of Medical Genetics
2020;37(9):938-941
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the correlation between microRNA (miRNA) differential expression and quality of embryo.
METHODS:The miRNA expression profiles of 8 blastocysts were detected by a TaqMan microRNA array, and miRNAs with a stable expression were selected. Additional blastocysts were selected, and the candidate miRNA was detected by real-time PCR. Meanwhile, chromosomal abnormalities of the embryos were detected by using next-generation sequencing, and the results were compared.
RESULTS:The expression of mir-720, mir-372, mir-886-3p and mir-512-3p was higher than that of miR-145, which suggested that mir-720, mir-372, mir-886-3p and mir-512-3p are related to early embryo development. The expression of miR-145 and mir-886-3p were significantly lower in the normal chromosome group. With the threshold values of above 9 and 3 for the relative expression of miR-145 and mir-886-3p, respectively, there was no embryo without a chromosomal abnormality.
CONCLUSION:There is a correlation between the expression level of specific miRNA and chromosomal abnormalities of embryos, which may be used as a novel biomarker for embryo selection.
