Analysis of miRNA-326's action on its target gene BCL-XL.
10.3760/cma.j.cn511374-20190918-00480
- Author:
Mingming QIAO
1
;
Xia GAI
;
Hui YE
;
Yanbo JI
;
Yuan YU
;
Yuanfeng CHEN
;
Huichong XU
;
Yunlong ZHUANG
Author Information
1. Blood Center of Shandong Province, Jinan, Shandong 250014, China. happymike110@126.com.
- Publication Type:Journal Article
- From:
Chinese Journal of Medical Genetics
2020;37(9):987-990
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the action of miRNA-326 on its target gene BCL-XL and the molecular mechanism of platelet apoptosis regulated by miRNAs.
METHODS:Dual-luciferase vectors containing respectively the wild-type and mutant 3'-untranslated region (3'UTR) fragments of the BCL-XL gene were constructed with firefly and renilla luciferases and transfected into 293T cells. Relative fluorescence intensities of the transfected cells were measured.
RESULTS:Dual-luciferase reporter gene vectors for PsiCHECK- BCL-XL -3'UTR-WT (wild-type) and PsiCHECK- BCL-XL -3' UTR-MT (variant) were respectively constructed. Relative fluorescence intensities of the 293T cells co-transfected by miRNA-326 and PsiCHECK- BCL-XL -3'UTR-WT plasmid were significantly lower compared with the control group (co-transfected by a miRNA-326 negative sequence and PsiCHECK- BCL-XL -3' UTR-WT plasmid) ( P = 0.034). The relative fluorescence intensity was also significantly reduced in cells co-transfected by miRNA-326 and PsiCHECK- BCL-XL -3' UTR-WT plasmid compared with the mutant control group co-transfected by miRNA-326 and PsiCHECK- BCL-XL -3'UTR-MT plasmid (P = 0.022).
CONCLUSION:miRNA-326 may participate in the regulation of platelet apoptosis by acting on the 3'-UTR of the BCL-XL gene.
