Application of in situ amniocyte culture for prenatal diagnosis.

Author: Daoqi HUANG ¹ ; Huayu TU ; Chaohong WANG ; Yaqun WANG ; Keting TONG ; Jiansheng ZHU
Author Information

1. Center for Genetic Medicine, Anhui Maternal and Child Health Care Hospital Affiliated to Anhui Medical University, Hefei, Anhui 230011, China. 593130772@qq.com.
Publication Type:Journal Article
From: Chinese Journal of Medical Genetics 2020;37(10):1117-1119
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To explore the value of in situ amniocyte culture for prenatal diagnosis.
METHODS:2716 amniotic fluid samples were cultured in situ on slides. After the culture, the slides were stained, photographed and analyzed.
RESULTS:All samples were successfully analyzed, with the success rates for primary culture and subculture being 98.42% and 1.58%, respectively. 224 samples (8.25%) were detected with chromosomal aberrations, which included 125 cases with trisomy 21, 31 with trisomy 18, 3 with trisomy 13, 4 with 45,X, 17 with 47,XXY, 5 with 47,XYY, 1 with 48,XXY,+18, 1 with 48,XXYY, 26 with structural chromosomal aberrations, and 11 with mosaicisms for aneuploidies.
CONCLUSION:In situ amniocyte culture is stable and has a high success rate, and is capable of identifying true and false mosaicisms, which can improve the accuracy of prenatal diagnosis.