A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae.
10.3343/kjlm.2010.30.1.34
- Author:
Borum SUH
1
;
Jin Kyoung CHUN
;
Dongeun YONG
;
Yang Soon LEE
;
Seok Hoon JEONG
;
Woo Ick YANG
;
Dong Soo KIM
Author Information
1. Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. deyong@yuhs.ac
- Publication Type:Brief Communication ; Case Reports
- Keywords:
Bartonella henselae;
Cat scratch disease;
Polymerase chain reaction
- MeSH:
Animals;
Bartonella henselae/genetics/*isolation & purification;
Cat-Scratch Disease/complications/*diagnosis;
Cats;
Child;
Dogs;
Female;
Humans;
Lymphadenitis/complications;
*Polymerase Chain Reaction;
RNA, Ribosomal, 16S/*genetics;
RNA, Ribosomal, 23S/*genetics;
Republic of Korea;
Sequence Analysis, DNA
- From:The Korean Journal of Laboratory Medicine
2010;30(1):34-37
- CountryRepublic of Korea
- Language:English
-
Abstract:
We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.
