Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo.

Author: Kai BA ¹ ; Duan NI ² ; Xin-Bo WANG ¹ ; Xue-Qin WEI ¹ ; Na LI ¹ ; Lian ZHENG ¹
Author Information

1. Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
2. Dept. of Emergency Medicine, Armed Police Corps Hospital of Henan, Zhengzhou 450000, China.
Publication Type:Journal Article
Keywords: cartilage regeneration; chondrocytes; cocultures; stromal vascular fraction
MeSH: Adipose Tissue; Animals; Cartilage; Chondrocytes; Coculture Techniques; Rabbits; Regeneration
From: West China Journal of Stomatology 2020;38(3):240-244
CountryChina
Language:Chinese
Abstract: OBJECTIVE:This study aimed to compare the cartilage regeneration of the stromal vascular fraction (SVF) cells and adipose-derived mesenchymal stem cells (ASCs) cocultured with chondrocytes seeded on the scaffolds.
METHODS:The cellular morphologies and proliferation capabilities on the scaffolds were evaluated. The scaffolds with the cocul-ture of ASCs/SVF and chondrocytes were implanted into the full thickness cartilage defective rabbit joints for 10 weeks.
RESULTS:The cells seeded into the scaffolds showed good adhesion and proliferation. Implantation with SVF and chondrocytes revealed desirable in vitro healing outcomes.
CONCLUSIONS:The SVF cells were better than ASCs in terms of the formation of cartilage matrix in a coimplantation model. Without in vitro expansion, the SVF cells are good cell sources for cartilage repair.