Effect of hot water extract of Korean ginseng on neuroblastoma cell parthanatos.
10.12122/j.issn.1673-4254.2020.09.14
- Author:
Yuanbo GUO
1
;
Dengwen ZHANG
1
;
Ruichun LONG
1
;
Songgui SHAN
2
;
Qiang SUN
1
;
Bin CAI
1
;
Sheng WANG
1
Author Information
1. Department of Anesthesiology, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
2. Department of Science and Education, Guangdong Provincial People's Hospital/Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
- Publication Type:Journal Article
- Keywords:
Korean ginseng;
apoptosis-inducing factor;
hot water extract;
parthanatos;
poly(ADP-ribose);
reactive oxygen species
- From:
Journal of Southern Medical University
2020;40(9):1313-1318
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of pretreatment of neuroblastoma cells with hot water extract of Korean ginseng on MNNG-induced parthanatos and its mechanism.
METHODS:Neuroblastoma SH-SY5Y cells were pretreated with 1 mg/L hot water extract of Korean ginseng before induction with 250 μmol/L MNNG for 1 h or 4 h. CCK-8 and cell flow cytometry were used to detect cell survival rate. Western blotting was used to detect the changes in poly(ADP-ribose) (PAR) expression in the treated cells. Immunofluorescence assay was used to detect nuclear distribution of apoptosis-inducing factor (AIF), and flow cytometry was used to detect the level of reactive oxygen species (ROS) in the cells.
RESULTS:Compared with the blank control cells, MNNG-treated SH-SY5Y cells showed significantly decreased survival rate as the concentration of MNNG and the stimulation time increased ( < 0.05). Stimulation with MNNG also resulted in significantly increased expression of PAR protein in the cells ( < 0.05). Pretreatment of the cells with hot water extract of Korean ginseng obviously inhibited MNNG-induced cell death and significantly reduced AIF expression and nucleation in the cells ( < 0.05). MNNG stimulation significantly increased ROS level in the cells, which was decreased significantly by pretreatment of the cells with the extract ( < 0.05).
CONCLUSIONS:Pretreatment with hot water extract of Korean ginseng reduces MNNG-induced parthanatos and ROS production in SH-SY5Y cells.
