Effect of nanohydroxyapatite on surface mineralization in acid-etched dentinal tubules and adsorption of lead ions.
10.12122/j.issn.1673-4254.2020.09.13
- Author:
Jianzhen YANG
1
;
Peiyan YUAN
1
;
Chengxia LIU
1
;
Ping LIU
1
;
Huili NING
1
;
Pingping XU
1
Author Information
1. Department of Endodontics, Stomatological Hospital, Southern Medical University/Guangdong Provincial Hospital of Stomatology, Guangzhou 510280, China.
- Publication Type:Journal Article
- Keywords:
adsorption;
dentin hypersensitivity;
lead ions;
nanohydroxyapatite;
surface mineralization
- From:
Journal of Southern Medical University
2020;40(9):1307-1312
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect of nanohydroxyapatite (nHA) for promoting surface mineralization of demineralized dentin discs and adsorption of lead ions in simulated sewage water.
METHODS:Sixty dentin disks were prepared from freshly extracted teeth with intact crown (including 30 premolars and 30 molars) and treated with 10% citric acid for 2 min to simulate dentinal tubules with dentin hypersensitivity. The etched dentin discs were brushed with distilled water, 0.2 g HA or 0.2 g nHA for 2 min twice a day for 7 consecutive days, after which scanning electron microscopy (SEM) was performed and calcium and phosphorus contents in the dentin discs were detected using EDS. Lead ion adsorption capacities of HA and nHA were tested by mixing 1 mL serial concentrations of HA and nHA suspensions with 50 mL lead ion solutions (1.0 mg/L). After 24 h, the residual lead ion concentration in the supernatant was measured using ICP to calculate lead ion adsorption rate and adsorption capacity of the materials.
RESULTS:SEM showed a smooth surface and empty dentin tubules in the acid- etched dentin dics. The dentin dics treated with HA were covered with masses of particles that loosely attached to the surface, and the diameter of the dentin tubules was reduced. In nHA group, the dentin discs showed a fine and homogeneous surface clogging with a tight attachment, and the dentin tubule diameter was obviously reduced. Daily brushing with HA and nHA, especially the latter, significantly increased calcium and phosphorus contents on the surface of the dentin dics ( < 0.000). In lead ion adsorption experiment, the lead ion adsorption rate of HA decreased as its concentration increased with the highest adsorption rate of 83.01%; the adsorption rate of nHA increased with its concentration until the adsorption equilibrium was reached, and its highest adsorption rate was 98.79%. A good liner relationship was found between the adsorption ability and concentration of HA.
CONCLUSIONS:Compared with HA, nHA has a better capacity for surface mineralization of acid-etched dentin discs and also a better ability of lead ion adsorption.
