Modified Xiaochaihu Decoction () Promotes Collagen Degradation and Inhibits Pancreatic Fibrosis in Chronic Pancreatitis Rats.
10.1007/s11655-017-2413-0
- Author:
Shu-Kun ZHANG
1
;
Nai-Qiang CUI
2
;
Yu-Zhen ZHUO
3
;
Jian-Gong HU
4
;
Jun-Hong LIU
5
;
Di-Hua LI
5
;
Li-Hua CUI
3
Author Information
1. Department of Cell and Molecular Biology, Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin, 300100, China. shu1971@163.com.
2. Department of the First Biliary and Pancreatic Surgery, Tianjin Nankai Hospital, Tianjin, 300100, China.
3. Department of Cell and Molecular Biology, Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin, 300100, China.
4. Department of Pathology, the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300150, China.
5. Department of Pharmacological, Institute of Acute Abdominal Diseases of Intergrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin, 300100, China.
- Publication Type:Journal Article
- Keywords:
Modified Xiaochaihu Decoction;
chronic pancreatitis rats;
collagen degradation;
matrix metalloproteinase 13;
tissue inhibitor of metalloproteinase 1
- From:
Chinese journal of integrative medicine
2020;26(8):599-603
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of Modified Xiaochaihu Decoction (MXD, ) on collagen degradation in rats with chronic pancreatitis (CP).
METHODS:Rats were injected dibutyltin dichloride (DBTC, 7 mg/kg of body weight) into the right caudal vein to induce CP model. Thirty heallhy male Wistar rats were randomly divided into three groups by a random number table: the control, the model and the treatment groups. Rats of treatment group were administered MXD (10 g/kg of body weight) orally once daily starting from the day post-model establishment. Pancreatic tissues were harvested after 28-day feeding and fibrosis was evaluated by picro-sirius red staining. The contents of collagen type I and III were detected using enzymelinked immunosorbent assay (ELISA), the expression of matrix metalloproteinase 13 (MMP13) and tissue inhibitor of metalloproteinase 1 (TIMP1) was analyzed by Western blot and real-time polymerase chain reaction (PCR).
RESULTS:The fibrosis scoring of pancreatic tissues, the concentrations of collagen type I and III, the expression levels of MMP13 and TIMP1 proteins and mRNA in the model group were all increased compared with the control group (P<0.05). After treatment with MXD, the fibrosis scoring of pancreatic tissues, the concentrations of collagen type I and III, the expression levels of MMP13 proteins and mRNA in the teatment group were all decreased compared with the model group (P<0.05), but there were no significant differences in the expression levels of TIMP1 proteins and mRNA (P>0.05).
CONCLUSIONS:MXD could promote collagen degradation and reverse pancreatic fibrosis in CP rats via a mechanism involve up-regulation of MMP13 expression.
