Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats.
10.1007/s11655-020-3183-7
- Author:
Hua-Bin ZHANG
1
;
Xian-Kun TU
2
;
Shi-Wei SONG
1
;
Ri-Sheng LIANG
1
;
Song-Sheng SHI
1
Author Information
1. Department of Neurosurgery, Fujian Medical University Union Hospital, Neurosurgery Research Institute of Fujian Province, Fuzhou, 350001, China.
2. Department of Neurosurgery, Fujian Medical University Union Hospital, Neurosurgery Research Institute of Fujian Province, Fuzhou, 350001, China. unionnstu@hotmail.com.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
aquaporin 4;
baicalin;
early brain injury;
matrix metalloproteinase-9;
subarachnoid hemorrhage
- From:
Chinese journal of integrative medicine
2020;26(7):510-518
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To evaluate the effect of baicalin on subarachnoid hemorrhage (SAH) in rats and explore the potential mechanisms.
METHODS:Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), myeloperoxidase (MPO), and malondialdehyde (MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2 (Nrf2), NAD(P)H: quinone oxidoreductase 1 (NQO1), matrix metalloproteinase-9 (MMP-9), aquaporin 4 (AQP4), occludin, and zonulaoccludens-1 (ZO-1) were detected in the brain by Western blot. Heme oxygenase-1 (HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were assessed by enzyme-linked immunosorbent assay.
RESULTS:Baicalin attenuated EBI 24 h after SAH in rats (P<0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats (P<0.05 or P<0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β (P<0.05 or P<0.01).
CONCLUSION:Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.
