Silence of cytoskeleton-associated protein 2 represses cell proliferation and migration and promotes apoptosis in liver cancer cell lines.
10.11817/j.issn.1672-7347.2020.180798
- Author:
Changsheng ZHANG
1
;
Xuezhen ZHANG
2
;
Zongming HAN
2
;
Hongbo ZHU
2
;
Tao WAN
3
Author Information
1. Department of General Surgery, Kaifeng Central Hospital, Kaifeng Henan 475000. 648318405@qq.com.
2. Department of General Surgery, Kaifeng Central Hospital, Kaifeng Henan 475000.
3. Department of Hepatobiliary Surgery, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China. wantaobj@163.com.
- Publication Type:Journal Article
- Keywords:
apoptosis;
cytoskeleton-associated protein 2;
liver cancer;
migration;
proliferation
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Cytoskeleton;
Humans;
Liver Neoplasms;
genetics
- From:
Journal of Central South University(Medical Sciences)
2020;45(4):365-371
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the roles of cytoskeleton-associated protein 2 (CKAP2) in proliferation, apoptosis, and migration in liver cancer cells and the potential mechanisms.
METHODS:Human normal hepatocyte L02 and liver cancer cell lines HepG2, Huh7, and SMMC-7721 were cultured. The CKAP2 expression was detected by real-time PCR and Western blotting. HepG2 cells were randomly divided into a control group, a negative control (NC) group, and a CKAP2 silencing (siCKAP2) group. CCK-8 and BrdU assays were used to evaluate cell viability and proliferation, respectively. Transwell assay was employed to determine cell migration and invasion. The protein levels of cleaved-caspase 3, Bax, E-cadherin, N-cadherin, Vimentin, phosphorylated Janus kinase 2 (p-JAK2), and phosphorylated signal transducer and activator of transcription 3 (p-STAT3) were determined by Western blotting.
RESULTS:Compared with normal hepatocyte L02, CKAP2 was highly expressed in liver cancer cell lines HepG2, Huh7, and SMMC-7721 (all <0.05). Compared with the NC group, cell viability and proliferation rate of the siCKAP2 group were decreased (both <0.05). The apoptotic rate, protein expression of cleaved-caspase 3 and Bax in the siCKAP2 group were significantly higher than those in the NC group (all <0.05). Compared with the NC group, cell migration and invasion rates of the siCKAP2 group were significantly attenuated (both <0.05). Compared with the NC group, E-cadherin protein expression in siCKAP2 group was increased, while protein expression levels of Vimentin, N-cadherin, p-JAK2, and p-STAT3 were decreased (all <0.05).
CONCLUSIONS:CKAP2 gene silence inhibits proliferation, migration, and invasion, and promotes apoptosis in liver cancer cells, while JAK2/STAT3 signaling pathway may be involved in these processes.
