The ethyl acetate extraction of Pileostegia tomentella (ZLTE) exerts anti-cancer effects on H1299 cells via ROS-induced canonical apoptosis.
10.1016/S1875-5364(20)30061-3
- Author:
Qiu-Mei FAN
1
,
2
;
Wen-Tong ZHAO
3
;
Renyikun YUAN
3
;
Qin-Qin WANG
1
,
2
;
Li-Feng ZHANG
4
;
Hong-Wei GAO
1
,
5
;
Jing LENG
6
,
7
;
Shi-Lin YANG
1
,
2
Author Information
1. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530000, China
2. Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning 530200, China.
3. State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
4. Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, Nanning, Guangxi 530200, China.
5. Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development, Nanning 530200, China. Electronic address: gaohongwei06@126.com.
6. Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, Nanning, Guangxi 530200, China
7. Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Nanning 530200, China. Electronic address: jingleng.gx@hotmail.com.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Lung cancer;
Pileostegia tomentella;
ROS
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(7):508-516
- CountryChina
- Language:English
-
Abstract:
Lung cancer is the leading cause of cancer death and the most common malignant tumor, the long-term survival of which has stagnated in the past several decades. Pileostegia tomentella Hand. Mazz is a traditional Chinese medicine called "Zhongliuteng" (ZLT) in the pharmacopeia, which has been proved to possess a potent anti-tumor effect on various cancers. In this study, the effects of ZLT N-butanol extraction (ZLTN) and ZLT ethyl acetate extraction (ZLTE) on the viability of non-small cell lung cancer cell (NSCLC) lines H1299 and A549 were evaluated. Here, we firstly reported that ZLTE significantly inhibited H1299 cells growth without affecting the release of lactate dehydrogenase (LDH). In addition, ZLTE induced caspase-dependent apoptosis in a concentration-dependent manner and increased the expression cleaved-PARP and decreased pro-caspase-3, pro-caspase-7, pro-caspase-8, and pro-caspase-9. Moreover, ZLTE increased the level of cellular reactive oxygen species (ROS) in H1299 cells to lead to apoptosis, which was reversed by N-acetyl-cysteine (NAC). Taken together, our results revealed that ZLTE induced caspase-dependent apoptosis via ROS generation, suggesting that ZLTE is a promising herbal medicine for the treatment of NSCLC.
