Molecular structure and phylogenetic analyses of the complete chloroplast genomes of three original species of Pyrrosiae Folium.
10.1016/S1875-5364(20)30069-8
- Author:
Chu-Hong YANG
1
,
2
;
Xia LIU
3
;
Ying-Xian CUI
1
,
4
;
Li-Ping NIE
1
,
4
;
Yu-Lin LIN
5
;
Xue-Ping WEI
5
;
Yu WANG
1
,
6
;
Hui YAO
1
,
7
Author Information
1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
2. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
3. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China.
4. Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China.
5. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
6. Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China. Electronic address: ywang@implad.ac.cn.
7. Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China. Electronic address: scauyaoh@sina.com.
- Publication Type:Journal Article
- Keywords:
Complete chloroplast genome;
Identification;
Phylogenetic relationship;
Pyrrosia lingua;
Pyrrosia petiolosa;
Pyrrosia sheareri
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(8):573-581
- CountryChina
- Language:English
-
Abstract:
Pyrrosia petiolosa, Pyrrosia lingua and Pyrrosia sheareri are recorded as original plants of Pyrrosiae Folium (PF) and commonly used as Chinese herbal medicines. Due to the similar morphological features of PF and its adulterants, common DNA barcodes cannot accurately distinguish PF species. Knowledge of the chloroplast (cp) genome is widely used in species identification, molecular marker and phylogenetic analyses. Herein, we determined the complete cp genomes of three original species of PF via high-throughput sequencing technologies. The three cp genomes exhibited a typical quadripartite structure with sizes ranging from 158 165 to 163 026 bp. The cp genomes of P. petiolosa and P. lingua encoded 130 genes, whilst that of P. sheareri encoded 131 genes. The complete cp genomes were compared, and five highly divergent regions of petA-psbJ, matK-rps16, ndhC-trnM, psbM-petN and psaC-ndhE were screened as potential DNA barcodes for identification of Pyrrosia genus species. The phylogenetic tree we obtained indicated that P. petiolosa and P. lingua are clustered in a single clade and, thus, share a close relationship. This study provides invaluable information for further studies on the species identification, taxonomy and phylogeny of Pyrrosia genus species.
