Genome-wide identification and analysis of AP2/ERF transcription factors related to camptothecin biosynthesis in Camptotheca acuminata.
10.1016/S1875-5364(20)30070-4
- Author:
Ya-Ting HU
1
,
2
,
3
;
Zhi-Chao XU
3
,
4
;
Ya TIAN
3
,
4
;
Ran-Ran GAO
3
,
4
;
Ai-Jia JI
3
,
4
;
Xiang-Dong PU
3
,
4
;
Yu WANG
3
,
4
;
Xia LIU
5
;
Jing-Yuan SONG
4
,
6
Author Information
1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
3. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China.
4. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
5. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China. Electronic address: lrx1125@126.com.
6. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China. Electronic address: jysong@implad.ac.cn.
- Publication Type:Journal Article
- Keywords:
AP2/ERF transcription factors;
CPT biosynthesis;
Camptotheca acuminata;
Expression pattern analysis;
Phylogenetic analysis
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(8):582-593
- CountryChina
- Language:English
-
Abstract:
Camptotheca acuminata produces camptothecin (CPT), a monoterpene indole alkaloid (MIA) that is widely used in the treatment of lung, colorectal, cervical, and ovarian cancers. Its biosynthesis pathway has attracted significant attention, but the regulation of CPT biosynthesis by the APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors (TFs) remains unclear. In this study, a systematic analysis of the AP2/ERF TFs family in C. acuminata was performed, including phylogeny, gene structure, conserved motifs, and gene expression profiles in different tissues and organs (immature bark, cotyledons, young flower, immature fruit, mature fruit, mature leaf, roots, upper stem, and lower stem) of C. acuminata. A total of 198 AP2/ERF genes were identified and divided into five relatively conserved subfamilies, including AP2 (26 genes), DREB (61 genes), ERF (92 genes), RAV (18 genes), and Soloist (one gene). The combination of gene expression patterns in different C. acuminata tissues and organs, the phylogenetic tree, the co-expression analysis with biosynthetic genes, and the analysis of promoter sequences of key enzymes genes involved in CPT biosynthesis pathways revealed that eight AP2/ERF TFs in C. acuminata might be involved in CPT synthesis regulation, which exhibit relatively high expression levels in the upper stem or immature bark. Among these, four genes (CacAP2/ERF123, CacAP2/ERF125, CacAP2/ERF126, and CacAP2/ERF127) belong to the ERF-B2 subgroup; two genes (CacAP2/ERF149 and CacAP2/ERF152) belong to the ERF-B3 subgroup; and two more genes (CacAP2/ERF095 and CacAP2/ERF096) belong to the DREB-A6 subgroup. These results provide a foundation for future functional characterization of the AP2/ERF genes to enhance the biosynthesis of CPT compounds of C. acuminata.
