Genome-wide identification of abscisic acid (ABA) receptor pyrabactin resistance 1-like protein (PYL) family members and expression analysis of PYL genes in response to different concentrations of ABA stress in Glycyrrhiza uralensis.
10.1016/S1875-5364(20)30072-8
- Author:
Ying-Xian CUI
1
,
2
;
Zhi-Chao XU
1
,
2
;
Xin-Lian CHEN
1
,
2
;
Li-Ping NIE
1
,
2
;
Li-Wei WU
1
,
2
;
Yu WANG
1
,
2
;
Jing-Yuan SONG
1
,
2
;
Hui YAO
1
,
3
Author Information
1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
2. Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China.
3. Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China. Electronic address: scauyaoh@sina.com.
- Publication Type:Journal Article
- Keywords:
Abscisic acid;
Gene expression;
Glycyrrhiza uralensis;
Pyrabactin resistance 1-like (PYR/PYL) protein family;
Signaling pathway;
Stress responses
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(8):606-611
- CountryChina
- Language:English
-
Abstract:
As abscisic acid (ABA) receptor, the pyrabactin resistance 1-like (PYR/PYL) protein (named PYL for simplicity) plays an important part to unveil the signal transduction of ABA and its regulatory mechanisms. Glycyrrhiza uralensis, a drought-tolerant medicinal plant, is a good model for the mechanism analysis of ABA response and active compound biosynthesis. However, knowledge about PYL family in G. uralensis remains largely unknown. Here, 10 PYLs were identified in G. uralensis genome. Characterization analysis indicated that PYLs in G. uralensis (GuPYLs) are relatively conserved. Phylogenetic analysis showed that GuPYL1-3 belongs to subfamily I, GuPYL4-6 and GuPYL10 belong to subfamily II and GuPYL7-9 belongs to subfamily III. In addition, transcriptome data presented various expression levels of GuPYLs under different exogenous ABA stresses. The expression pattern of GuPYLs was verified by Quantitative real-time polymerase chain reaction (qRT-PCR). The study proved that GuPYL4, GuPYL5, GuPYL8 and GuPYL9 genes are significantly up-regulated by ABA stress and the response process is dynamic. This study paves the way for elucidating the regulation mechanism of ABA signal to secondary metabolites and improving the cultivation and quality of G. uralensis using agricultural strategies.
