Correlation of the Expression Alteration of Tim-3 with the T Cell and B Cell Dysfunction in Peripheral Blood of Multiple Myeloma Patients.
10.19746/j.cnki.issn.1009-2137.2020.03.027
- Author:
Hui LIU
1
;
Jian-Qiang ZHAO
2
;
Zeng-Feng NI
2
;
Yu-Hong LIU
3
;
Jiao JIAO
2
Author Information
1. Department of Hematology, The First Affiliated Hospital of Xi'an Medical College, Xi'an 710021, Shaanxi Province, China,E-mail: liuhui_509@163.com.
2. Department of Hematology, The First Affiliated Hospital of Xi'an Medical College, Xi'an 710021, Shaanxi Province, China.
3. Department of Immune Rheumatism, Affiliated Hospital of Yan'an University, Yanan 716000, Shaanxi Province, China.
- Publication Type:Journal Article
- MeSH:
B-Lymphocytes;
CD8-Positive T-Lymphocytes;
Hepatitis A Virus Cellular Receptor 2;
metabolism;
Humans;
Leukocytes, Mononuclear;
Multiple Myeloma
- From:
Journal of Experimental Hematology
2020;28(3):881-885
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the correlation of the expression alteration of Tim-3 with the T cell and B cell dysfunction in peripheral blood of multiple myeloma (MM) patients.
METHODS:30 patients diagnosed as MM from October 2016 to October 2018 were selected and enrolled in MM group, and 30 healthy persons whose sex and age was matched with the MM patients were selected and enrolled in healthy control group (HC). The blood samples from MM patients and HC were collected, and the peripheral blood mononuclear cells (PBMNC) were separated by density gradient centrifugation, then the serum was kept for further study. The ratios of CD3CD4Tim-3T cells, CD3CD8Tim-3T cells and the CD19+CD20-CD38+B cells were analysed by flow cytometry (FCM)，and the concentration of T cell-related cytokines IFN-γ, TNF-αand B cell-related antibodies IgA, IgM and IgG were measured by ELISA. At the same time, the differences of the ratios of CCD3CD4Tim-3T, CD3CD8Tim-3T cells and plasmablast and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG between the MM patient and HC were estimated, and the correlation of the ratio of CD3CD4Tim-3T, CD3CD8Tim-3T cells with the ratio of plasmablast and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG in MM patients were analyzed.
RESULTS:The ratio of CD3CD4Tim-3T, CD3CD8Tim-3T cells increased in MM patients, while the ratio of CD19+CD20- CD38+B cells and the concentration of IFN-γ, TNF-α, IgA, IgM and IgG decreased in MM patients. And there was a negative correlation of the ratio of CD3CD4Tim-3T cells with CD19+CD20-CD38+B cells and the concentration of IFN-γ, IgA, IgM and IgG in MM patients, while the ratio of CD3CD8Tim-3T cells just negatively correlated with the concentration of TNF-α.
CONCLUSION:Expression of Tim-3 on CD4 and CD8 cells elevates in the peripheral blood of MM patients, which also correlates with the function suppression of T and B cells.
