Effect of Protein Kinase A Activation on Aggregation Function of Platelets.
10.19746/j.cnki.issn.1009-2137.2020.03.030
- Author:
Meng-Xiao JIANG
1
;
Jun LIU
1
;
Kang-Xi ZHOU
1
;
Hong-Lei YE
1
;
Ren-Ping HU
1
;
Rong YAN
1
;
Chang-Geng RUAN
1
;
Ke-Sheng DAI
2
Author Information
1. Medical College of Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University and Collaborative Innovation Center of Hematology, State Key Laboraotry of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, Jiangsu Province, China.
2. Medical College of Soochow University, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University and Collaborative Innovation Center of Hematology, State Key Laboraotry of Radiation Medicine and Protection, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, Jiangsu Province, China,E-mail: kdai@suda.edu.cn.
- Publication Type:Journal Article
- MeSH:
Blood Platelets;
Cyclic AMP-Dependent Protein Kinases;
Humans;
Platelet Aggregation;
Platelet Aggregation Inhibitors;
Ristocetin;
Thrombin
- From:
Journal of Experimental Hematology
2020;28(3):899-903
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of protein kinase A (PKA) activation on aggregation funetion of platelets in vitro.
METHODS:The peripheral blood of healthy adults were collected, and the washed platelets were gained from collected peripheral blood. The washed platelets were treated with PKA activator Forskolin, then the platelet aggregation was induced by using Ristocetin, Thrombin, Collagen and ADP respectively, the platelet aggregation level was detected by the platelet aggregator.
RESULTS:Compared with the controls, 5 μmol/L forskolin significantly inhibited ADP and collagen-induced platelet aggregation (P＜0.001), and showed mild inhibiting effect on Thrombin-induced platelet aggregation (P＜0.05). 2.5-10 μmol/L forskolin significantly inhibited ADP and Collagen -induced platelet aggregation (P＜0.001); but not showed significantly inhibitory effects on Ristocetin-induced platelet aggregation (P＞0.05).
CONCLUSION:PKA activation inhibits agonists-induced platelet aggregation.
