Effects of Different Routes in PMSC Transfusion on the Levels of Hematopoietic Regulatory Factors in Aplastic Anemia Rats.
10.19746/j.cnki.issn.1009-2137.2020.03.036
- Author:
Chuan-Ming LIN
1
;
Li-Ping LIU
1
;
Shui-Ling XIE
1
;
Wen-Hong LAI
1
;
Xi XU
1
;
Cai-Dong HU
1
;
Chang-Feng LIAO
1
;
Xiao-Yu CHEN
1
;
Hai-Liang LI
2
Author Information
1. Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China.
2. Department of Hematology, The First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China,E-mail：gyfylhl@163.com.
- Publication Type:Journal Article
- MeSH:
Anemia, Aplastic;
Animals;
Female;
Hematopoietic Stem Cell Transplantation;
Interferon-gamma;
Mesenchymal Stem Cells;
Placenta;
Pregnancy;
Rats;
Tumor Necrosis Factor-alpha
- From:
Journal of Experimental Hematology
2020;28(3):937-941
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of different routes in placental mesenchymal stem cells (PMSC) on serum expression levels of IL-4, IL-17, TNF-α and IFN-γ in aplastic anemia (AA) rats.
METHODS:The rat model of aplastic anemia (AA rats) was established by 5-fluorouracil combined with busulfan. The rats was divided into four groups: control, experimental, PMSC-injected into the tail vein, and PMSC-injected into the medullary cavity. The general state of rats in each group was observed in detail before and after treatment. The serum levels of interleukin-4 (IL-4) , interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) were measured by enzyme-linked immunosorbent assay (ELISA) at week 1, 3 and 5 after treatment.
RESULTS:The serum levels of TNF-α, IFN-γ and IL-17 in the experimental group were significantly higher than those in the control group, while the level of IL-4 was significantly decreased (P＜0.05). The levels of TNF-α, IFN-γ and IL-17 gradually decreased after treatment while the level of IL-4 increased. By the fifth week, the above indexes were closed to the control group (P＞0.05), and the levels of TNF-α, IFN-γ and IL-17 in the group with PMSCs injected via the medullary cavity decrease more significantly than those group with PMSC injected via the tail vein, but level of IL-4 was not significantly different between two groups.
CONCLUSION:The level of serum hematopoietic negative regulators increase significantly, and the level of hematopoietic promoting factors decreases significantly in aplastic anemia rats. PMSC can down-regulate the level of hematopoietic negative regulators and up-regulate the level of hematopoietic promoting factors in the rats with aplastic anemia, and the inhibition of hematopoietic negative regulators by intramedullary injection is more significant than that by caudal vein injection.
