Development and Application of Automatic Analysis and Surveillance Platform for Chrimerism in Donors and Recipients after Allo-HSCT.
10.19746/j.cnki.issn.1009-2137.2020.03.049
- Author:
Jian-Cheng FANG
1
;
Fang WANG
1
;
Li-Li YUAN
1
;
Mian-Mian WANG
1
;
Ting-Ting LI
1
;
Yi-Hang YANG
1
;
Yang LIU
1
;
Xiao-Li MA
1
;
Xue CHEN
1
;
Yang ZHANG
1
;
Dai-Jing NIE
1
;
Jia-Qi CHEN
1
;
Hong-Xing LIU
2
Author Information
1. Department of Internal Medicine Teaching and Research Section of Henan Medical College, Zhengzhou 451191, Henan Province, China.
2. Department of Internal Medicine Teaching and Research Section of Henan Medical College, Zhengzhou 451191, Henan Province, China,Beijing LU Dao-Pei Institute of Hematology, Beijing 100176, China,Department of Pathology & Laboratorial Medicine, Beijing LU Dao-Pei Hospital, Beijing 100176, China,E-mail: starliu@pku.edu.cn.
- Publication Type:Journal Article
- MeSH:
Electrophoresis, Capillary;
Genotype;
Hematopoietic Stem Cell Transplantation;
Humans;
Microsatellite Repeats;
Tissue Donors
- From:
Journal of Experimental Hematology
2020;28(3):1012-1018
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an automated chimeric analysis and reporting platform based on short tandem repeat (STR) and capillary electrophoresis methods for allogeneic hematopoietic stem cell transplantation (allo-HSCT) so as to improve work efficiency.
METHODS:Apache, MySQL, PHP and HTML5 were used to build the database and interface. The STR locus geno typing and chimeric analysis logic and flow were set up on the basis of STR rules and capillary electrophoresis. STR genotyping and 194 times of chimeric testing data of 100 patients after allo-HSCT were used to test the platform for automatic STR locus genotyping, chimeric calculation and report generation.
RESULTS:The established platform could realize the functions of STR locus customization, STR genotype determination, automatic chimeric analysis, and detection information database management, which can automatically generate an integrated report including multiple sequential chimeric results and trend graphs for the same patient and can be accessed and used simultaneously by different users through different browser interfaces. The results of automated analysis by the platform are completely consistent with that of manual analysis by experienced technicians, and the possibility of manual analysis error is reduced through automation. The time required for automatic analysis using this platform is approximately 1/6-1/5 of manual analysis.
CONCLUSION:The automatic analysis platform built in this study is operation stable and reliable in analysis results, which can improve work efficiency and report connotation, thus worthing popularized and applicable.
