Effect of Mmu-circRNA_016901 on the Radiosensitivity of Bone Marrow Mesenchymal Stem Cells.
10.19746/j.cnki.issn.1009-2137.2020.03.052
- Author:
Jun-Hua ZHANG
1
;
Xian-Hui WEN
1
;
Rong HUANG
1
;
Sai CHEN
1
;
Feng-Xia LIU
1
;
Rong GUI
2
Author Information
1. Department of Blood Transfusion, The Third Yiangya Hospital of Central South University, Changsha 41000, Hunan Province, China.
2. Department of Blood Transfusion, The Third Yiangya Hospital of Central South University, Changsha 41000, Hunan Province, China,E-mail: aguirong@163.com.
- Publication Type:Journal Article
- MeSH:
Bone Marrow Cells;
Mesenchymal Stem Cells;
RNA, Circular;
genetics;
Radiation Tolerance
- From:
Journal of Experimental Hematology
2020;28(3):1032-1037
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of mmu-circRNA_016901 on the regulation of radiation injury of bone marrow stem cells and its mechanism.
METHODS:Bone marrow stem cells were exposed to different dose of X-ray, then the expression level of mmu-circRNA_016901 in bone marrow cells treated with different doses of X-ray was detected. The luciferase reporter gene assay was used to confirm that miRNA1249-5p is the target of mmu-circRNA_016901, and RNA Binding Protein Immunoprecipitation was used to confirm that TGF-β3 is the targeted on miRNA1249-5p，the expression of TGF-β3 and cell proliferation were detected after the expression of mmu-circRNA_01690 was regulated.
RESULTS:When the irradiation dose＜6 Gy, there were significant difference in the expression of mmn-circRNA-016901 after the bone marrow mesenchymal stem cells were treated by different doses of irradiation, which showed a statistically significant (P＜0.05). The luciferase reporter gene detection and co-immunoprecipitation experiments confirmed that Mmu-circRNA_016901 could binds to miRNA1249-5p specifically, and overexpression of mmu-circRNA_016901 could regulate miRNA1249-5p negatively, which resulted in a significant increase in TGF-β3 expression and promoting of cell proliferation.
CONCLUSION:mmu-circRNA_016901 affects the expression of TGF-β3 through miRNA1249-5p, and thus participates in the regulation of the radiation damage mechanism of bone marrow mesenchymal stem cells.
