Effect of Chidamide on the Killing Acitivity of NK Cells Targeting K562 Cells and Its Related Mechanism In Vitro.
10.19746/j.cnki.issn.1009-2137.2020.04.014
- Author:
Liang-Yin WENG
1
;
Lei XUE
2
;
Cai-Xia HE
1
;
Qian-Wen XU
1
;
Cui-Ying CHU
3
;
You-Ming WANG
3
;
Xing-Bing WANG
4
Author Information
1. Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230000, Anhui Province, China.
2. Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230000, Anhui Province, China,Department of Hematology, The First Affiliated Hospital, University of Science and Technology of China,Hefei 230000, Anhui Province, China.
3. College of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230000, Anhui Province, China.
4. Department of Hematology, Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230000, Anhui Province, China,Department of Hematology, The First Affiliated Hospital, University of Science and Technology of China, Hefei 230000, Anhui Province, China,E-mail:wangxingbing@ustc.edu.cn.
- Publication Type:Journal Article
- MeSH:
Aminopyridines;
Benzamides;
GPI-Linked Proteins;
Histocompatibility Antigens Class I;
Humans;
Intercellular Signaling Peptides and Proteins;
K562 Cells;
Killer Cells, Natural;
immunology;
NK Cell Lectin-Like Receptor Subfamily K
- From:
Journal of Experimental Hematology
2020;28(4):1167-1170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of chidamide on the killing activity of NK (Natural killer cell, NK) cells targeting K562 cells and its related mechanism.
METHODS:K562 cells were pretreated with chidamide at different concentrations and cocultured with NK cells at different effect-target ratios. The killing effect of chidamide on K562 cells by NK cells, the expression of natural killer group 2 member D (NKG2D) ligands and apoptosis rate of K562 cells were detected by flow cytometry.
RESULTS:The killing sensitivity of NK cells to K562 cells could be enhanced by chidamide. The expression of ULBP2 on K562 cell surface could be up-regulate, however, the expression of ULBP1 and MICA/MICB showed no statistically difference as compared with control group. Chidamide showed no obvious cytotoxicity to K562 cells.
CONCLUSION:Chidamide can significantly improve killing efficiency of NK cells on K562 cells, which may be related to the up-regulation of ULBP2 expression.
