Mechanism of Hematopoietic Reconstruction in Mice Treated with Danggui Buxue Decoction combined with Muscle-Derived Stem Cell Transplantation.
10.19746/j.cnki.issn.1009-2137.2020.04.016
- Author:
Hao-Ying DOU
1
;
Ya-Ge DU
1
;
Yuan-Hao HAN
2
;
Yuan-Yuan WANG
1
;
Xiao-Ling WANG
2
;
Tao WANG
3
Author Information
1. Department of Surgery and Gynecology, Nursing College of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
2. Department of Histology and Embryology Integrated Chinese and Western Medicine College of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
3. Department of Histology and Embryology Integrated Chinese and Western Medicine College of Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China,E-mail：wangtao6112@ 163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
Female;
Hematopoietic Stem Cell Transplantation;
Hematopoietic System;
Mice;
Spleen
- From:
Journal of Experimental Hematology
2020;28(4):1177-1182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the mechanism of hematopoietic reconstruction in mice treated with Danggui Buxue Decoction (DBD) combined with the muscle-derived stem cell transplantation (MDSCT).
METHODS:Female Kunming mice were randomly divided into the 6 groups: irradiation model, the bone marrow transplantation, the MDSC transplantation, the DBD 1 (4.5 g/kg), 2 (13.5 g/kg), and 3 (22.5 g/kg) + MDSC transplantation. After a week of oral administration of normal saline or different doses of DBD, The mice were exposied to 8 Gy Cs γ ray and were followed by bone marrow or MDSC transplantation. The expression levels of Notch1, Jagged1 and Hes1 in bone marrow, thymus and spleen were measured at 3 and 8 weeks after irradiation and transplantation.
RESULTS:In the bone marrow, 3 weeks after above-mentioned treatment, the expression of Notch1 mRNA increased obviously and the expression of Jagged1, Hes1 mRNA decreased obviously in each intervention group, compared with the irradiation model group. 8th week after treatment, the expression of Notch1 mRNA decreased obviously in each intervention group, the Jagged1 mRNA expression decreased obviously except the bone marrow group, and Hes1 mRNA expression increased (P＜0.05) in each intervention group. 3 weeks after treatment, compared with the irradiation model group, the expression of Notch1 mRNA in the thymocytes increased only in DBD1+MDSC group, Jagged1, Hes1 mRNA was increased in the MDSC transplantation group and the DBD1、2+MDSC group. 8th week after treatment, the expression of Notch1, Jagged1 mRNA expression decreased in each intervention group, the expression of Hes1 mRNA increased obviously in the MDSC transplantation group and the DBD1、2+MDSC group (P＜0.05). In the spleen, 3 weeks after treatment, the expression of Notch1, Jagged1 mRNA in the spleen of each intervention group decreased obviously, compared with the irradiation model group. The expression of Jagged1, Hes1 mRNA in each intervention group were increased obviously 8th week after treatment (P＜0.05).
CONCLUSION:MDSC transplantation after pretreatment of DBD can improve the hematopoietic reconstitution in mice with lethal dose radiation damage. Notch1、Jagged1 and Hes1 play different roles in this process, but the concrete mechanism needs to be further studied.
