MiR-144 Inhibits the Malignant Biologic Behaviors of Multiple Myeloma Cells by Regulating Wnt4/β-Catenin Signaling Pathway.
10.19746/j.cnki.issn.1009-2137.2020.04.029
- Author:
Liang WANG
1
;
Min XU
1
;
Jian XING
1
;
Zong-Hua HUANG
1
;
Guo-Qiang LIU
2
Author Information
1. Department. of Hematology, Shengli Oilfield Center Hospital, Dongying 257034, Shandong Province, China.
2. Department. of Hematology, Shengli Oilfield Center Hospital, Dongying 257034, Shandong Province, China,E-mail:18676022931@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Biological Products;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Humans;
MicroRNAs;
Multiple Myeloma;
Wnt Signaling Pathway;
Wnt4 Protein;
beta Catenin
- From:
Journal of Experimental Hematology
2020;28(4):1256-1260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of miR-144 to the biological behavior of multiple myeloma cells and its mechanism.
METHODS:RT-PCR was used to detect the expression of miR-144 in multiple myeloma cells and plasma of MM patients. MTT assay was used to detect the proliferation and cloning ability of myeloma cells transfected by miR-144. Flow cytometry was used to detect the cell cycle distribution of myeloma cells with over-expression of miR-144. Apoptosis of myeloma cells with over-expression of miR-144 was detected by TUNEL assay. Transwell cell invasion and migration assay was used to detect the invasion and migration ability of myeloma cells with overexpressing on miR-144.Western blot analysis was used to detect the protein expression levels of MMP-9 and MMP-2 in myeloma cells with over expression of miR-144, as well as the expression levels of proteins related to Wnt/β-catenin signaling pathway.
RESULTS:The expression level of miR-144 in MM cell lines and blood of MM patients was significantly lower than that in control group (P＜0.05). The proliferation, invasion and migration of myeloma cells with over-expression of miR-144 were significantly decreased (P＜0.05), and the apoptosis level was increased (P＜0.05). The expression levels of MMP-9, MMP-2, Wnt/β-catenin signaling pathway in myeloma cells with over-expression of miR-144 were significantly lower than those in control group (P＜0.05).
CONCLUSION:MiR-144 can inhibit the proliferation, migration and invasion of multiple myeloma cells and induce cell apoptosis. The specific mechanism may be related with the activity of inhibiting Wnt/β-catenin signaling pathway.
