Effect of Met Knockdown on Biological Behavior of Multiple Myeloma RPMI 8226 Cells.
10.19746/j.cnki.issn.1009-2137.2020.04.033
- Author:
Hui-Li LIU
1
;
Jie SHANGGUAN
2
;
Wen-Zhong QUE
3
Author Information
1. Department of Medical Technology, ZhangZhou Health Vocational College, /Collaborative Innovation Center for Translation Medical Testing and Application Technology, Zhangzhou 363000, Fujian Province, China.
2. Department of Anesthesiology, The Affiliated Nanping First Hospital of Fujian Medical University, Nanping 353000, Fujian Province, China.
3. Department of Rheumatology, The Affiliated Nanping First Hospital of Fujian Medical University, Nanping 353000, Fujian Province, China,E-mail：quewenzhong@163.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Multiple Myeloma;
RNA, Small Interfering
- From:
Journal of Experimental Hematology
2020;28(4):1278-1282
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of down-regulating of c-Met expression to the proliferation, invasiveness and apoptosis of human multiple myeloma RPMI 8226 cells.
METHODS:According to transfection the RPMI8226 cells were dividide into RPMI 8226 (untreated RPMI 8226), RPMI 8226 /shRNA-Met and RPMI8226/shRNA-control group, respectively. Protein expression level of c-Met was detected by Western blot so as to evaluate transfection condition; the proliferation of the cells was detected by MTT; apoptosis and cycle of the cells were detected by flow cytometry; effect of c-Met/shRNA on RPMI 8226 cell adhesion was detected by RPMI 8226 cell adherence to ECM (Fn and Matrigel) and ECV304 cells. Invasiveness of RPMI 8226 cell was detected by Transwell assay.
RESULTS:The c-Met short hairpin RNA (shRNA) was successfully transfected into RPMI 8226 cells, and could inhibit the expression of c-Met significantly. The down-regulation of c-Met could inhibit the proliferation of RPMI 8226 cells significantly. The percentage of cells in the G/G phase and apoptotic rate (sub-G) in the RPMI 8226/shRNA-Met group were higher than those in the control group, the adhesion rate and the number of migrated RPMI 8226/shRNA-Met cells were decreased significantly as compared with control group. There were no significant differences in each indexes between RPMI 8226/shRNA-control and control group.
CONCLUSION:Knockdown of c-Met can affect the proliferation, adherence, invasiveness and apoptosis of human multiple myeloma RPMI 8226 cells.
