Effect of Sonic Hedgehog Signal Pathway Inhibitor Jervine on Myelodysplastic Syndromes MUTZ-1 Cells.

Yu-ting Qin; Liu Yao; Zhen Yin; Huan Wang; Shuang Chen; Tuerxun Nilupar; Yasen Halida; Yang Liu; Abulizi Patiguli; Ming Jiang; Jian-ping Hao

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Effect of Sonic Hedgehog Signal Pathway Inhibitor Jervine on Myelodysplastic Syndromes MUTZ-1 Cells.

Author: Yu-Ting QIN ^{1
,

2} ; Liu YAO ^{1
,

2} ; Zhen YIN ^{1
,

2} ; Huan WANG ^{1
,

2} ; Shuang CHEN ^{1
,

2} ; Tuerxun NILUPAR ^{1
,

2} ; Yasen HALIDA ^{1
,

2} ; Yang LIU ^{1
,

2} ; Abulizi PATIGULI ^{1
,

2} ; Ming JIANG ^{1
,

2} ; Jian-Ping HAO ^{1
,

3}
Author Information

1. Hematologic Disease Center, The First Affiliated Hospital of Xinjiang Medical University
2. Xinjiang Uygur Autonomous Region Research Institute of Hematology, Urumqi 830061, Xinjiang Uygur Autonomous Region, China.
3. Xinjiang Uygur Autonomous Region Research Institute of Hematology, Urumqi 830061, Xinjiang Uygur Autonomous Region, China,E-mail: 13579876416@163.com.
Publication Type:Journal Article
MeSH: Apoptosis; Cell Line, Tumor; Cell Proliferation; Hedgehog Proteins; Humans; Myelodysplastic Syndromes; Signal Transduction; Veratrum Alkaloids
From: Journal of Experimental Hematology 2020;28(4):1298-1302
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To study the effect of SMO inhibitor (Jervine) on proliferation, apoptosis and cell cycle of MDS cell line MUTZ-1, and its mechanism.
METHODS:The effect of different concentrations Jervine on proliferation of MUTZ-1 cells was detected by CCK-8 method. Apoptosis and cell cycle of MUTZ-1 cells were detected by flow cytometry. Western blot was used to detect the changes of Shh signaling pathway effecting proteins BCL2 and CyclinD1. The expression levels of Smo and Gli1 gene were detected by real-time fluorescent quantitative polymerase chain reaction (RT-qPCR).
RESULTS:Jervine inhibited MUTZ-1 cell proliferation in a concentration dependent manner (24 h, r=-0.977), the apoptosis rate of MUTZ-1 cells increased with the enhancement of concentration of Jervine in MUTZ-1 cells (P＜0.001), the cell proportion of G phase increased and the cell number of S phase decreased with enhancement of concentration (P＜0.001). The result of RT-qPCR and Western blot showed that the expression of Smo, Gli1 mRNA and BCL2, CyclinD1 proteins decreased (P＜0.05).
CONCLUSION:SMO inhibitor can effectively inhibit the growth of MDS cell line MUTZ-1 improve the cell apoptosis and induce cell cycle arrest. Its action mechanism may be related with dowm-regulating the expression of BCL2 and CyclinD1.