PDGFRα Cell-Derived SCF Regulates Hematopoiesis of Adult Mice.
10.19746/j.cnki.issn.1009-2137.2020.04.046
- Author:
Zhao-Feng ZHENG
1
,
2
,
3
;
Fang-Lin GOU
4
;
Tao CHENG
1
,
2
,
5
;
Hui CHENG
1
,
2
,
6
Author Information
1. State Key Laboratory of Experimental Hematology
2. Tianjin 300070, China,Institute of Hematology & Blood Diseases Hospital，Chinese Academy of Medical Sciences & Peking Union Medical College
3. Tianjin 300070, China.
4. School of Basic Medical Sciences，Tianjin Medical University，Tianjin 300070, China.
5. Tianjin 300070, China,Center for Stem Cell Medicine, Chinese Academy of Medical Sciences,Tianjin 300070, China,Department of Stem Cell & Regenerative Medicine, Peking Union Medical College，Tianjin 300020, China,National Clinical Research Center for Blood Disease, Tianjin 300020, China.
6. Tianjin 300070, China,Center for Stem Cell Medicine, Chinese Academy of Medical Sciences,Tianjin 300070, China,Department of Stem Cell & Regenerative Medicine, Peking Union Medical College，Tianjin 300020, China,National Clinical Research Center for Blood Disease, Tianjin 300020, China,E-mail: chenghui@ihcams.ac.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow;
Bone Marrow Cells;
Hematopoiesis;
Mice;
Receptor, Platelet-Derived Growth Factor alpha;
Stem Cell Factor
- From:
Journal of Experimental Hematology
2020;28(4):1349-1356
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of PDGFRα stromal cells derived SCF on hematopoiesis of adult mice.
METHODS:Pdgfrα-CreER; R26-tdTomato mice model was constructed, and the proportion and distribution of PDGFRα cells in the liver, spleen, lung, kidney and bone marrow were analyzed by flow cytometry and confocal microscopy. Then the Pdgfrα-CreER; Scf mice model was further constructed, the Scf in PDGFRα was knocked out specifically, the effect of Scf-knocked out in PDGFRα stromal cells in the propitiation of HSPCs in the bone marrow was analyzed by flow cytometry. The effect of SCF on the proportion on number of peripheral blood cells in mice was analyzed by whole blood analyzer.
RESULTS:After Scf was knocked out in PDGFRα stromal cells, the propitiation and number of LKS- cell, LKS+ cell, HSC, MPP1, MKP, PreGM, PreMegE, and CFU-E in the bone marrow of mice was decreased, as well as in the number of red blood cells and hemoglobin concentration of peripheral blood. However, Scf knocked out from PDGFRα cells showed no effect on the hematopoiesis in spleen.
CONCLUSION:specific knocked out of Scf in PDGFRα stromal cells in adult mice can decrease the proportion of HSPCs in the bone marrow and the number of red blood cells in peripheral blood, and finally lead to anemia in mice.
