Effect of Korean Magnolia obovata Extract on Platelet-Derived Growth Factor-Induced Vascular Smooth Muscle Cells.
10.1007/s11655-019-3171-y
- Author:
Hyunjhung JHUN
1
;
Suji BAEK
2
;
Jinwoo KIM
3
;
Kang-Pa LEE
3
;
Hun-Young PARK
4
;
Won-Hwan PARK
5
;
Kiwon LIM
4
;
Jisu KIM
6
Author Information
1. Technical Assistance Center, Korea Food Research Institute, Jeonbuk, 55365, Republic of Korea.
2. Department of Medical Science, School of Medicine, Konkuk University, Seoul, Republic of Korea.
3. Department of Bio-Science, College of Natural Science, Dongguk University, Gyeongbuk, 38066, Republic of Korea.
4. Physical Activity & Performance Institute, Konkuk University, Seoul, 05029, Republic of Korea.
5. Department of Diagnostic, College of Korean Medicine, Dongguk University Goyang, Gyeonggi-do, 10326, Republic of Korea.
6. Physical Activity & Performance Institute, Konkuk University, Seoul, 05029, Republic of Korea. kimpro@konkuk.ac.kr.
- Publication Type:Journal Article
- Keywords:
Akt;
extracellular signal-regulated protein kinases 1 and 2;
functional food;
platelet-derived growth factor-BB;
smooth muscle cell
- From:
Chinese journal of integrative medicine
2020;26(9):677-682
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effects of Korean Magnolia obovata crude extract (KME) on plateletderived growth factor (PDGF)-BB-induced proliferation and migration of vascular smooth muscle cells (VSMCs).
METHODS:KME composition was analyzed by high-performance liquid chromatography (HPLC). VSMCs were isolated from the aorta of a Sprague-Dawley rat, incubated in serum free-Dulbecco's modified Eagle's medium in the presence or absence of KME (10, 30, 100, and 300 μg/mL), then further treated with PDGF-BB (10 ng/mL). VSMC proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and VSMC migration was determined using the Boyden chamber and scratch wound healing assays. Western blot analysis was used to detect phosphorylation of extracellular signal-regulated protein kinases 1 and 2 (p-ERK1/2), protein kinase B (p-Akt), and stress-activated protein kinase/c-Jun NH2-terminal kinase (p-SAPK/JNK). The antimigration and proliferation effects of KME were tested using aortic sprout outgrowth.
RESULTS:The HPLC analysis identified honokiol (0.45 mg/g) and magnolol (0.34 mg/g) as the major components of KME. KME (30, 100, and 300 μg/mL) significantly decreased the proliferation and migration of PDGF-BB-stimulated (10 ng/mL) VSMCs and the PDGF-BB-induced phosphorylation of EKR1/2, Akt, and SAPK/JNK (P<0.05). Furthermore, PDGF-BBinduced VSMCs treated with 300 μg/mL of KME showed reduction in aortic sprout outgrowth.
CONCLUSION:KME could inhibit abnormal proliferation and migration of VSMCs by down-regulating the phosphorylation of EKR1/2 and Akt. Thus, KME might be a functional food for preventing vascular disorders.
