Inhibitory Mechanisms of Water Extract of Oplopanax elatus on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Murine Macrophage Cells.
10.1007/s11655-020-3188-2
- Author:
Ki Sun KWON
1
;
Hyun LIM
1
;
Yong Soo KWON
1
;
Myong Jo KIM
2
;
Ji Hye YOO
3
;
Nam Ho YOO
2
;
Hyun Pyo KIM
4
Author Information
1. College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of Korea.
2. Department of Bio-Resource Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea.
3. Bioherb Research Institute, Kangwon National University, Chuncheon, 24341, Republic of Korea.
4. College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of Korea. hpkim@kangwon.ac.kr.
- Publication Type:Journal Article
- Keywords:
Chinese medicine;
Oplopanax elatus;
RAW 264.7;
anti-inflammation;
stem;
syringin
- From:
Chinese journal of integrative medicine
2020;26(9):670-676
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To study the anti-inflammatory action and cellular mechanism of Oplopanax elatus.
METHODS:A hot water extract of OE (WOE) was prepared and a major constituent, syringin, was successfully isolated. Its content in WOE was found to be 214.0 µg/g dried plant (w/w). Their anti-inflammatory activities were examined using RAW 264.7 macrophages and a mouse model of croton oil-induced ear edema.
RESULTS:In lipopolysaccharide (LPS)-treated RAW 264.7 cells, a mouse macrophage cell line, WOE was found to significantly and strongly inhibit cyclooxygenase-2 (COX-2)-induced prostaglandin E (PGE) production [half maximal inhibitory concentration (IC)=135.2 µg/mL] and inducible nitric oxide synthase (iNOS)-induced NO production (IC=242.9 µg/mL). In the same condition, WOE was revealed to inhibit NO production by down-regulating iNOS expression, mainly by interrupting mitogen activated protein kinases (MAPKs)/activator protein-1 (AP-1) pathway. The activation of all three major MAPKs, p38 MAPK, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase, was inhibited by WOE (50-300 µg/mL). On the other hand, WOE reduced PGE production by inhibiting COX-2 enzyme activity, but did not affect COX-2 expression levels. In addition, WOE inhibited the production of proinflammatory cytokines such as interleukin-6 and tumor necrosis factor-α. In croton oil-induced ear edema in mice, oral administration of WOE (50-300 mg/kg) dose-dependently inhibited edematic inflammation.
CONCLUSION:Water extract of OE exhibited multiple anti-inflammatory action mechanisms and may have potential for treating inflammatory disorders.
