Aerobic exercise reduces the expression of pyroptosis-related proteins and inflammatory factors in hippocampus of mice with insulin resistance.
- Author:
Xue-Peng BIAN
1
;
Rui-Fang JI
1
;
Bei-Bei LIU
1
;
Jing-Yun HU
1
;
Ming-Ming LI
1
;
Xiang-Li XUE
1
;
Shu-Jie LOU
2
Author Information
1. School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China.
2. School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China. shujielou319@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Caspase 1;
Gene Expression;
Hippocampus;
Inflammasomes;
Insulin Resistance;
Male;
Mice;
Mice, Inbred C57BL;
NIMA-Related Kinases;
NLR Family, Pyrin Domain-Containing 3 Protein;
Physical Conditioning, Animal;
Pyroptosis
- From:
Acta Physiologica Sinica
2020;72(4):455-462
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to observe the expression of pyroptosis- and inflammation-related proteins in the hippocampus of mice with insulin resistance (IR) after aerobic exercise, and to explore the possible mechanism of exercise to improve IR. C57BL/6J male mice of 6 weeks old were randomly fed with normal diet (n = 12) and high-fat diet (HFD) (n = 26) for 12 weeks respectively. Glucose tolerance test (GTT) and insulin tolerance test (ITT) were performed to determine whether IR occurred in HFD mice. Then the mice were randomly divided into control group (n = 12), IR group (n = 10) and IR + aerobic exercise group (AE, n = 10). Mice in AE group performed a 12-week progressive speed treadmill training after being adapted to the treadmill for one week. After the intervention, the expression of pyroptosis- and inflammation-related proteins in hippocampus was detected by Western blot. The results showed that compared with control group, NFκB, Nod-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing CARD (ASC), pyroptosis-related proteins like pro-Caspase-1, gasdermin D (GSDMD), GSDMD-N, and inflammatory factors IL-1β, IL-18 were significantly increased. The inflammasome-related protein NIMA-related kinase 7 (NEK7) and pyroptosis-related protein Caspase-1 showed an increasing trend, but there was no significant difference. Compared with the IR group, progressive speed treadmill training significantly reduced the expression of NFκB, NLRP3, NEK7, ASC, pro-Caspase-1, GSDMD, GSDMD-N, IL-1β, and IL-18 in the hippocampus of mice with IR. These results suggested 12-week progressive speed treadmill training can significantly reduce the expression of pyroptosis-related proteins and inflammatory factors in the hippocampus of mice with IR, and inhibit pyroptosis.
