In Vivo Two-photon Calcium Imaging in Dendrites of Rabies Virus-labeled V1 Corticothalamic Neurons.
10.1007/s12264-019-00452-y
- Author:
Yajie TANG
1
;
Liang LI
2
;
Leqiang SUN
2
;
Jinsong YU
2
;
Zhe HU
2
;
Kaiqi LIAN
1
;
Gang CAO
2
;
Jinxia DAI
3
Author Information
1. Academician Workstation of Animal Disease Control and Nutrition Immunity in Henan Province, Henan Joint International Research Laboratory of Veterinary Biologics Research and Application, Anyang Institute of Technology, Anyang, 455000, China.
2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
3. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China. jxdai@mail.hzau.edu.cn.
- Publication Type:Journal Article
- Keywords:
Corticothalamic projection;
Cranial window;
Dendrite;
In vivo Ca2+ imaging;
Neural circuit tracing;
Primary visual cortex;
Rabies virus;
Two-photon microscopy
- From:
Neuroscience Bulletin
2020;36(5):545-553
- CountryChina
- Language:English
-
Abstract:
Monitoring neuronal activity in vivo is critical to understanding the physiological or pathological functions of the brain. Two-photon Ca imaging in vivo using a cranial window and specific neuronal labeling enables real-time, in situ, and long-term imaging of the living brain. Here, we constructed a recombinant rabies virus containing the Ca indicator GCaMP6s along with the fluorescent protein DsRed2 as a baseline reference to ensure GCaMP6s signal reliability. This functional tracer was applied to retrogradely label specific V1-thalamus circuits and detect spontaneous Ca activity in the dendrites of V1 corticothalamic neurons by in vivo two-photon Ca imaging. Notably, we were able to record single-spine spontaneous Ca activity in specific circuits. Distinct spontaneous Ca dynamics in dendrites of V1 corticothalamic neurons were found for different V1-thalamus circuits. Our method can be applied to monitor Ca dynamics in specific input circuits in vivo, and contribute to functional studies of defined neural circuits and the dissection of functional circuit connections.
