Construction and function of a root-specific promoter SRSP.

Wenwen Cui; Jing Chi; Yanfang Feng; Lili Geng; Rongmei Liu

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Construction and function of a root-specific promoter SRSP.

Author: Wenwen CUI ¹ ; Jing CHI ² ; Yanfang FENG ¹ ; Lili GENG ² ; Rongmei LIU ¹
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1. School of Life Sciences, Northeast Agricultural University, Harbin 150000, Heilongjiang, China.
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Publication Type:Journal Article
Keywords: cis-acting elements; gus; root-specific promoter; synthetic promoter
MeSH: Agrobacterium; genetics; Cloning, Molecular; Gene Expression Regulation, Plant; Plant Roots; genetics; Plants, Genetically Modified; Promoter Regions, Genetic; genetics; Stress, Physiological; Tobacco; genetics; growth & development; Transformation, Genetic
From: Chinese Journal of Biotechnology 2020;36(4):700-706
CountryChina
Language:Chinese
Abstract: The responsibility of root is absorbing water and nutrients, it is an important plant tissue, but easily to be affected by biotic and abiotic stresses, affecting crop growth and yield. The design of a synthetic root-specific promoter provides candidate promoters for the functional analysis and efficient expression of stress-related genes in crop roots. In this study, a synthetic root-specific module (pro-SRS) was designed using tandem four-copies of root specific cis-acting elements (OSE1ROOTNODULE, OSE2ROOTNODULE, SP8BFIBSP8AIB, and ROOTMOTIFAPOX1), and fused with minimal promoter from the CaMV 35S promoter to synthesize an artificially synthetic SRSP promoter. The SRSP promoter was cloned in pCAMBIA2300.1 by replacing CaMV 35S promoter so as to drive GUS expression. The constructs with SRSP promoter were transformed in tobacco by Agrobacterium-mediated method. SRSP promoter conferred root-specific expression in transgenic tobacco plants through Real-time PCR (RT-PCR) analysis and GUS histochemical staining analysis. It is indicated that the repeated arrangement of cis-acting elements can realize the expected function of the promoter. This study laid a theoretical foundation for the rational design of tissue-specific promoters.