Disruption of OsRhoGDI2 by CRISPR/Cas9 technology leads to semi-dwarf in rice.

Kaijie Wang; Wenjing AN; Yafei Liu; Di Liu; Lianjie Feng; Junjie Wang; Junjun Huang; Xiaofei Liu; Weihong Liang

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Disruption of OsRhoGDI2 by CRISPR/Cas9 technology leads to semi-dwarf in rice.

Author: Kaijie WANG ¹ ; Wenjing AN ¹ ; Yafei LIU ¹ ; Di LIU ¹ ; Lianjie FENG ¹ ; Junjie WANG ¹ ; Junjun HUANG ¹ ; Xiaofei LIU ¹ ; Weihong LIANG ¹
Author Information

1. College of Life Sciences, Henan Normal University, Xinxiang 453007, Henan, China.
Publication Type:Journal Article
Keywords: CRISPR/Cas9 technology; OsRhoGDI2; plant height; rice
MeSH: CRISPR-Cas Systems; Genes, Plant; genetics; Oryza; genetics; growth & development; Plants, Genetically Modified; rho Guanine Nucleotide Dissociation Inhibitor beta; genetics
From: Chinese Journal of Biotechnology 2020;36(4):707-715
CountryChina
Language:Chinese
Abstract: OsRhoGDI2 was isolated as a putative partner of Rho protein family member OsRacD from rice panicles by yeast two-hybrid, but its function remains unknown. In order to identify the function of OsRhoGDI2, OsRhoGDI2 knockout mutants were created by CRISPR/Cas9 technology. The results showed that two different homozygous mutants were obtained in T0 generation, and eight kinds homozygous mutants were identified in T1 generation. Sequence analysis revealed that the base substitution or base deletion occurred near the editing targets of the gene in knockout rice, and it could be expected that the truncated OsRhoGDI2 proteins lacking the RhoGDI conserved domain would be generated. Phenotype analysis showed that the OsRhoGDI2 knockout rice plants were significantly lower than the control plants. Statistical analysis confirmed that the significant decrease of plant height was due to the shortening of the second and third internodes, suggesting that OsRhoGDI2 gene may be related with rice height control.