Research progress and application of nanopore sequencing technology.

Author: Ying CAO ¹ ; Wei LI ² ; Xin CHU ³ ; Ke WU ¹ ; Haizhou LIU ¹ ; Di LIU ¹
Author Information

1. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei, China.
2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
3. Bureau of Major R&D Programs, Chinese Academy of Sciences, Beijing 100864, China.
Publication Type:Journal Article
Keywords: animal genome; bacterial genome; clinical research; nanopore sequencing technology; plant genome; viral genome
MeSH: Animals; Base Sequence; DNA; chemistry; genetics; Humans; Nanopore Sequencing; trends; Nanopores; Research; trends; Sequence Analysis, DNA; trends
From: Chinese Journal of Biotechnology 2020;36(5):811-819
CountryChina
Language:Chinese
Abstract: Sequencing technology has been greatly improved in terms of throughput and cost. The single-molecule nanopore DNA sequencing, one of the major branches of the third-generation sequencing technology, has made great contributions in the fields of medicine and life sciences due to its advantages of ultra-long reading length, real-time detection and direct detection of base methylation modification, etc. This article briefly describes the principle of nanopore sequencing technology, and discusses its application in clinical, animal, plant, bacterial and virus fields and its future development direction.