Advances in gene editing of Corynebacterium glutamate.

Juanjuan Yang; Xiaoyu MA; Xiaorui Wang; Zhaohui Zhang; Shan Wang; Huimin Qin; Shuhong Mao; Fuping LU

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Advances in gene editing of Corynebacterium glutamate.

Author: Juanjuan YANG ¹ ; Xiaoyu MA ¹ ; Xiaorui WANG ¹ ; Zhaohui ZHANG ¹ ; Shan WANG ¹ ; Huimin QIN ¹ ; Shuhong MAO ¹ ; Fuping LU ¹
Author Information

1. School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300451, China.
Publication Type:Journal Article
Keywords: CRISPR; Corynebacterium glutamicum; gene editing; homologous recombination
MeSH: CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Corynebacterium glutamicum; genetics; Gene Editing; trends; Glutamic Acid; Industrial Microbiology; trends
From: Chinese Journal of Biotechnology 2020;36(5):820-828
CountryChina
Language:Chinese
Abstract: Corynebacterium glutamicum, an important microorganism to produce amino acids and organic acids, has been widely applied in food and medicine fields. Therefore, using editing tools to study the function of unknown genes in C. glutamicum has great significance for systematic development of industrial strain with efficient and novel production capability. Recently, gene editing has been greatly developed. Traditional gene editing based on homologous recombination and gene editing mediated by nuclease are successfully applied in C. glutamicum. Among these, the CRISPR system has been developed to be a main tool used for gene knockout of C. glutamicum due to its advantages of efficiency, simplicity and good target specificity. However, more efficient and reliable knockout system is still urgently demanded, to help develop high-performing strains in industrial application.