Effects of SCD-1 gene overexpression on the content of calcium ion and lipids in duck uterine epithelial cells.
- Author:
Jiezhang LI
1
;
Hualun LUO
1
;
Guanghui TAN
1
;
Lei WU
1
;
Yuanyu QIN
1
;
Yiyu ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Ca²⁺; SCD-1 gene; duck; lipid indexes; uterine epithelial cells
- MeSH: Animals; Calcium; metabolism; Coenzyme A Ligases; genetics; Ducks; Epithelial Cells; chemistry; enzymology; Gene Expression; Ions; Lipids; genetics; Triglycerides; metabolism
- From: Chinese Journal of Biotechnology 2020;36(5):899-907
- CountryChina
- Language:Chinese
- Abstract: Stearoyl-CoAdesaturase-1 (SCD-1) is a key regulator of monounsaturated fatty acid synthesis. It plays a vital role in lipid synthesis and metabolism. Ca²⁺ is an important cation in the body and plays an important role in the organism. The aims of this study were to investigate the correlation of SCD-1 gene overexpression with lipid indexes and calcium ion level. The pcDNA3.1 (+) + SCD-1 +Flag eukaryotic expression vector and cultured duck uterine epithelial cells were co-transfected. The overexpression of SCD-1 gene was measured using the Flag Label Detection Kit. Ca ions and lipid contents were detected through Fluo-3/AM Calcium Ion Fluorescence Labeling method and Lipid Measuring Kit, respectively. SCD-1 gene overexpression was negatively correlated with triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C), and positively correlated with Ca ion, total cholesterol (TC), very low-density lipoprotein cholesterol (VLDL-C) and low density lipoprotein cholesterol (LDL-C) levels. Meanwhile, Ca ion was positively correlated with TG, LDL-C and HDL-C contents, and negatively correlated with TC and VLDL-C levels. Overexpression of SCD-1 gene could regulate Ca ion secretion, as well as lipid synthesis and transport in duck uterine epithelial cells.
