Cloning, expression and characterization of a new endo-β-N-acetylglucosaminidase from Streptomyces alfalfae.

Author: Lingcong LI ¹ ; Shaofeng HU ¹ ; Tianyan GU ¹ ; Chenyin LÜ ¹ ; Yanchi LIU ¹ ; Hua LIU ¹ ; Jingang GU ² ; Guogang ZHAO ¹
Author Information

1. College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China.
2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Publication Type:Journal Article
Keywords: Streptomyces alfalfae; deglycosylation; endo-β-N-acetylglucosaminidase
MeSH: Cloning, Molecular; Enzyme Stability; Escherichia coli; genetics; Gene Expression; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; genetics; metabolism; Recombinant Proteins; genetics; metabolism; Streptomyces; enzymology; genetics
From: Chinese Journal of Biotechnology 2020;36(5):932-941
CountryChina
Language:Chinese
Abstract: Endo-β-N-acetylglucosaminidase is used widely in the glycobiology studies and industries. In this study, a new endo-β-N-acetylglucosaminidase, designated as Endo SA, was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21 (DE3). The purified recombinant Endo SA exhibited the maximum activity at 35 ºC and pH 6.0, good thermo/pH stability and high specific activity (1.0×10⁶ U/mg). It displayed deglycosylation activity towards different protein substrates. These good properties make EndoSA a potential tool enzyme and industrial biocatalyst.