Biocatalysis of formaldehyde to L-xylose.

Author: Zhailin CHU ¹ ; Xiaoyun LU ² ; Yuwan LIU ² ; Bo CUI ² ; Meidong JING ¹ ; Huifeng JIANG ²
Author Information

1. College of Life Sciences, Ludong University, Yantai 264025, Shandong, China.
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Publication Type:Journal Article
Keywords: L-xylose; biosynthesis; formaldehyde; formaldehyde reaction; glycolaldehyde
MeSH: Biocatalysis; Formaldehyde; chemistry; Fructose-Bisphosphate Aldolase; metabolism; Xylose; chemical synthesis
From: Chinese Journal of Biotechnology 2020;36(5):942-948
CountryChina
Language:Chinese
Abstract: It is of great significance to use biosynthesis to transform the inorganic substance formaldehyde into organic sugars. Most important in this process was to find a suitable catalyst combination to achieve the dimerization of formaldehyde. In a recent report, an engineered glycolaldehyde synthase was reported to catalyze this reaction. It could be combined with engineered D-fructose-6-phosphate aldolase, a "one-pot enzyme" method, to synthesize L-xylose using formaldehyde and the conversion rate could reach up to 64%. This process also provides a reference for the synthesis of other sugars. With the increasing consumption of non-renewable resources, it was of great significance to convert formaldehyde into sugar by biosynthesis.